Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science Block, 6th floor, Burma Colony, Karaikudi, Tamil Nadu, 630004, India.
Unit of Vector Control, Phytochemistry and Nanotechnology, Department of Zoology, Annamalai University, Annamalai Nagar, Tamil Nadu, 608 002, India.
Environ Sci Pollut Res Int. 2018 Jul;25(19):18604-18619. doi: 10.1007/s11356-018-2002-6. Epub 2018 Apr 27.
Microbial polysaccharides produced by marine species play a key role in food and cosmetic industry, as they are nontoxic and biodegradable polymers. This investigation reports the isolation of exopolysaccharide from Bacillus licheniformis Dahb1 and its biomedical applications. Bacillus licheniformis Dahb1 exopolysaccharide (Bl-EPS) was extracted using the ethanol precipitation method and structurally characterized. FTIR and H-NMR pointed out the presence of various functional groups and primary aromatic compounds, respectively. Bl-EPS exhibited strong antioxidant potential confirmed via DPPH radical, reducing power and superoxide anion scavenging assays. Microscopic analysis revealed that the antibiofilm activity of Bl-EPS (75 μg/ml) was higher against Gram-negative (Pseudomonas aeruginosa and Proteus vulgaris) bacteria over Gram-positive species (Bacillus subtilis and Bacillus pumilus). Bl-EPS led to biofilm inhibition against Candida albicans when tested at 75 μg/ml. The hemolytic assay showed low cytotoxicity of Bl-EPS at 5 mg/ml. Besides, Bl-EPS achieved LC values < 80 μg/ml against larvae of mosquito vectors Anopheles stephensi and Aedes aegypti. Overall, our findings pointed out the multipurpose bioactivity of Bl-EPS, which deserves further consideration for pharmaceutical, environmental and entomological applications.
海洋物种产生的微生物多糖在食品和化妆品工业中发挥着关键作用,因为它们是无毒且可生物降解的聚合物。本研究报告了从地衣芽孢杆菌 Dahb1 中分离胞外多糖及其在生物医学中的应用。采用乙醇沉淀法提取地衣芽孢杆菌 Dahb1 胞外多糖(Bl-EPS),并对其进行结构表征。FTIR 和 H-NMR 分别指出存在各种功能基团和主要芳香化合物。通过 DPPH 自由基、还原力和超氧阴离子清除实验证实了 Bl-EPS 具有较强的抗氧化潜力。显微镜分析表明,Bl-EPS(75μg/ml)对革兰氏阴性(铜绿假单胞菌和普通变形杆菌)细菌的抗生物膜活性高于革兰氏阳性菌(枯草芽孢杆菌和短小芽孢杆菌)。当在 75μg/ml 下测试时,Bl-EPS 对白色念珠菌的生物膜抑制作用。溶血实验表明,Bl-EPS 在 5mg/ml 时的细胞毒性较低。此外,Bl-EPS 对疟蚊传播媒介按蚊和埃及伊蚊的幼虫的 LC 值均小于 80μg/ml。总的来说,我们的研究结果指出了 Bl-EPS 的多功能生物活性,值得进一步考虑用于制药、环境和昆虫学应用。