Burlington D B, Djeu J Y, Wells M A, Kiley S C, Quinnan G V
J Immunol. 1984 Jun;132(6):3154-8.
We report that large granular lymphocytes (LGL) have an accessory function in the development of cytotoxic T cells (Tc) through the production of soluble factor(s). LGL and T cells were separated on Percoll gradients and the ability of the separated and of the recombined LGL and T cells to generate influenza A virus-specific Tc activity was measured. When stimulated by virus-infected, irradiated, adherent cells, neither LGL nor T cells cultured separately produced Tc activity. When they were co-cultured, however, even if separated by a 0.22-micron pore size membrane, Tc responses were readily generated from the small T cell precursors and natural killer activity was maintained in the LGL. Thus, LGL were required as accessory cells for Tc responses to occur and the effect was mediated by a soluble factor(s). alpha-Interferon (IFN) was produced in cultures containing LGL and/or stimulating adherent cells, whereas gamma-IFN was only produced in cultures containing both LGL and T cells. Therefore, neither alpha- nor gamma-IFN appeared to be the LGL produced soluble factor that mediated the accessory effect of LGL on Tc responses.
我们报告称，大颗粒淋巴细胞（LGL）通过产生可溶性因子在细胞毒性T细胞（Tc）的发育中具有辅助功能。在Percoll梯度上分离LGL和T细胞，并测定分离及重组后的LGL和T细胞产生甲型流感病毒特异性Tc活性的能力。当受到病毒感染、照射过的贴壁细胞刺激时，单独培养的LGL和T细胞均不产生Tc活性。然而，当它们共同培养时，即使被0.22微米孔径的膜隔开，也能从小的T细胞前体中轻易产生Tc反应，并且LGL中能维持自然杀伤活性。因此，Tc反应的发生需要LGL作为辅助细胞，且这种效应是由一种或多种可溶性因子介导的。α干扰素（IFN）在含有LGL和/或刺激贴壁细胞的培养物中产生，而γ干扰素仅在同时含有LGL和T细胞的培养物中产生。因此，α干扰素和γ干扰素似乎都不是LGL产生的介导LGL对Tc反应辅助作用的可溶性因子。
