Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208, Porto, Portugal.
Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208, Porto, Portugal.
Fish Shellfish Immunol. 2018 Jul;78:317-321. doi: 10.1016/j.fsi.2018.04.056. Epub 2018 Apr 25.
In aquaculture, animals can be continually exposed to environmental stress factors that put their health and even survival at risk. Two experiments were performed to evaluate the impact of different stress conditions (acute crowding and anaesthetic) on the natural haemolytic complement activity in serum and skin mucus of gilthead seabream (Sparus aurata L.). In the first experiment, fish were subjected to 10 kg m (low density, control group) and 50 kg m (high density, crowding group) during 2, 24 and 48 h. In the second experiment, fish were unexposed (control) or exposed to 40 ppm of MS-222 or 5 ppm or 10 ppm of clove oil for 1 h. In fish maintained in acute crowding conditions only an increase of the haemolytic complement activity was observed in the skin mucus after 24 h of exposure. However, a similar statistically significant increase was observed in serum and skin mucus of fish exposed for 1 h to the lowest concentration of clove oil (5 ppm) tested. The results point to a new and alternative way to assess stress in farmed fish by using skin mucus instead of blood serum and confirm that the measurement of natural haemolytic complement activity serves as an indicator of stress in fish.
在水产养殖中，动物可能会不断暴露于环境应激因素下，这会威胁到它们的健康甚至生存。进行了两项实验来评估不同应激条件（急性拥挤和麻醉）对真鲷（Sparus aurata L.）血清和皮肤黏液中天然溶血补体活性的影响。在第一个实验中，鱼在 2、24 和 48 小时内分别处于 10kg/m（低密度，对照组）和 50kg/m（高密度，拥挤组）的环境中。在第二个实验中，鱼处于未暴露（对照组）或暴露于 40ppm 的 MS-222 或 5ppm 或 10ppm 的丁香油 1 小时的环境中。在处于急性拥挤条件下的鱼中，仅在暴露 24 小时后观察到皮肤黏液中的溶血补体活性增加。然而，在血清和皮肤黏液中，暴露于测试的最低浓度（5ppm）丁香油 1 小时的鱼中也观察到了类似的统计学显著增加。这些结果为通过使用皮肤黏液而不是血清来评估养殖鱼类的应激提供了一种新的替代方法，并证实了天然溶血补体活性的测量可作为鱼类应激的指标。
