Canullo Luigi, Genova Tullio, Naenni Nadja, Nakajima Yasushi, Masuda Katsuhiko, Mussano Federico
Via Nizza, 46, 00198 Rome, Italy.
CIR Dental School, Department of Surgical Sciences UNITO, via Nizza 230, 10126 Turin, Italy.
Ann Anat. 2018 Jul;218:265-270. doi: 10.1016/j.aanat.2018.03.005. Epub 2018 Apr 26.
Plasma of argon treatment was demonstrated to increase material surface energy leading to stronger and faster interaction with cells. The aim of the present in vitro study was to test the effect of plasma treatment on different graft materials.
Synthetic hydroxyapatite (Mg-HA), biphasic calcium phosphate (BCP), cancellous and cortical xenogeneic bone matrices (CaBM, CoBM) were used representing commonly used classes of bone substitute materials. Fifty serially numbered disks with a 10mm-diameter from each graft material were randomly divided into two groups: test group (argon plasma treatment) and control group (absence of treatment). Cell morphology (using pre-osteoblastic murine cells) and protein adsorption were analyzed at all samples from both the test and control group. Differences between groups were analyzed using the Mann-Whitney test setting the level of significance at p<0.05.
Plasma treatment significantly increased the protein adsorption at all samples. Similarly, plasma treatment significantly increased cell adhesion in all groups.
Data confirmed that non-atmospheric plasma of argon treatment led to an increase of protein adsorption and cell adhesion in all groups of graft material to a similar extent.
Plasma of argon is able to improve the surface conditions of graft materials.
已证明氩气处理血浆可增加材料表面能，从而导致与细胞的相互作用更强、更快。本体外研究的目的是测试血浆处理对不同移植材料的影响。
使用合成羟基磷灰石（Mg-HA）、双相磷酸钙（BCP）、松质骨和皮质异种骨基质（CaBM、CoBM），它们代表了常用的骨替代材料类别。从每种移植材料中制备50个直径为10mm的连续编号圆盘，随机分为两组：试验组（氩等离子体处理）和对照组（未处理）。对试验组和对照组的所有样本分析细胞形态（使用前成骨细胞系小鼠细胞）和蛋白质吸附情况。使用Mann-Whitney检验分析组间差异，将显著性水平设定为p<0.05。
血浆处理显著增加了所有样本的蛋白质吸附。同样，血浆处理显著增加了所有组的细胞黏附。
数据证实，氩气非大气等离子体处理在所有移植材料组中均导致蛋白质吸附和细胞黏附程度相似地增加。
氩气等离子体能够改善移植材料的表面状况。
