用于蛋白质侧链动力学特性描述的 H 静磁场 NMR 基础实验。
Basic experiments in H static NMR for the characterization of protein side-chain dynamics.
机构信息
Department of Chemistry, University of Colorado Denver, Denver, CO 80204, USA.
Department of Mathematics, University of Colorado Denver, Denver, CO 80204, USA.
出版信息
Methods. 2018 Sep 15;148:136-145. doi: 10.1016/j.ymeth.2018.04.030. Epub 2018 Apr 27.
Abstract
The focus of this review is the basic methodology for applications of static deuteron NMR for studies of dynamics in the side chains of proteins. We review experimental approaches for the measurements of static line shapes and relaxation rates as well as signal enhancement strategies using the multiple echo acquisition scheme. Further, we describe computational strategies for modeling jump and diffusive motions underlying experimental data. Applications are chosen from studies of amyloid fibrils comprising the amyloid-β protein.
摘要
本文重点介绍了用于研究蛋白质侧链动力学的静态氘核 NMR 应用的基本方法。我们回顾了用于测量静态线谱和弛豫率的实验方法，以及使用多回波采集方案的信号增强策略。此外，我们还描述了用于对实验数据所基于的跳跃和扩散运动进行建模的计算策略。本文选择了包含淀粉样蛋白-β 蛋白的淀粉样纤维的研究作为应用实例。
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