Liu Dan, Huang Chuang, Xu Kong-Rong, Hu Jing, Lei Lin, Yuan Xiao-Bo, Fan Li-Qing, Zhu Wen-Bing
Research Institute of Reproduction and Stem Cell Engineering,Central South University, Changsha, Hunan 410008, China.
CITIC-Xiangya Hospital of Reproduction and Genetics, Central South University, Changsha, Hunan 410008, China.
Zhonghua Nan Ke Xue. 2017 Mar;23(3):231-236.
To investigate whether in vitro culture medium (IVCM) for sparse spermatozoa can improve human sperm motility for the purpose of helping clinicians, laboratorians and patients choose a better strategy of assisted reproduction.
Semen samples were obtained from 178 males for routine semen examination from March to August 2016, including 151 cases of asthenozoospermia and 27 cases of normal sperm motility. A total of 200 μl was collected from each sample and divided into two equal portions and equal volumes of IVCM (experimental group) and F10 (1×) (control group) were added to the two portions, respectively, followed by 30-minute incubation at 37℃ in an incubator with 5% CO2. Sperm concentration, motility and viability and the percentages of progressively motile, non-progressively motile and immotile sperm were recorded before and after incubation.
After activated with IVCM, neither the samples with asthenozoospermia nor those with normal sperm motility showed any statistically significant difference in sperm viability from the baseline or the control group (P>0.05). The rates of progressively and non-progressively motile sperm from the asthenozoospermia males were increased by 14.02% and 4.86% respectively, while that of immotile sperm decreased by 19.01% in the experimental group (P >0.01), and similar results were observed in the semen samples from the men with normal sperm motility. The percentage of reduced immotile viable sperm was positively correlated with that of immotile viable sperm in both the asthenozoospermia patients (r = 0.260, P <0.01) and the men with normal sperm motility (r = 0.679, P <0.01).
IVCM can increase sperm motility without affecting sperm viability in men with either asthenozoospermia or normal sperm motility. The larger the proportion of immotile viable sperm, the higher the percentages of progressively and non-progressively motile sperm in the semen after IVCM activation, and this correlation is more significant in men with normal sperm motility than in asthenozoospermia patients.
研究用于稀少精子的体外培养基（IVCM）能否提高人类精子活力，以帮助临床医生、检验人员和患者选择更好的辅助生殖策略。
2016年3月至8月，收集178名男性的精液样本进行常规精液检查，其中包括151例弱精子症患者和27例精子活力正常者。从每个样本中采集200μl精液并分成两等份，分别向两份中加入等体积的IVCM（实验组）和F10（1×）（对照组），然后在含5%二氧化碳的培养箱中于37℃孵育30分钟。记录孵育前后的精子浓度、活力、存活率以及进行性运动、非进行性运动和不运动精子的百分比。
用IVCM激活后，弱精子症样本和精子活力正常的样本在精子存活率方面与基线或对照组相比均无统计学显著差异（P>0.05）。实验组中，弱精子症男性的进行性和非进行性运动精子率分别提高了14.02%和4.86%，而不运动精子率降低了19.01%（P>0.01），精子活力正常男性的精液样本也观察到类似结果。在弱精子症患者（r = 0.260，P<0.01）和精子活力正常男性（r = 0.679，P<0.01）中，不运动存活精子减少的百分比与不运动存活精子的百分比均呈正相关。
IVCM可提高弱精子症或精子活力正常男性的精子活力，且不影响精子存活率。不运动存活精子的比例越大，IVCM激活后精液中进行性和非进行性运动精子的百分比越高，这种相关性在精子活力正常男性中比在弱精子症患者中更显著。
