Jang Hoon
Dept. of Biomedical Science, College of Life Sciences, CHA University, Seongnam 13488, Korea.
Dev Reprod. 2018 Mar;22(1):65-72. doi: 10.12717/DR.2018.22.1.065. Epub 2018 Mar 31.
Cyclic AMP-response element binding protein zhangfei (CREBZF), a member of ATF/CREB (activating transcription factor/ cAMP response element binding protein) family, regulates numerous cellular functions and development of cells by interacting transcription factors. This study discovered the expression pattern of CREBZF in seminiferous tubule of testes during the postnatal development of mice. In testis, CREBZF mRNA expression was the highest among other organs. Immunofluorescence analyses showed that the CREBZF was specifically expressed on spermatocyte but not in spermatogonia and Sertoli cells in seminiferous epithelium of mouse testis. Semi-quantitative polymerase chain reaction (PCR) analysis showed that CREBZF transcript level was significantly elevated during postnatal development of mouse testis. Confocal imaging analysis indicated that the protein expression of CREBZF in seminiferous tubule remained low until postnatal day (PD) 14, and was dramatically increased in PD 21. Interestingly, only one type of the spermatocyte expressed CREBZF specifically among SCP3-positive spermatocytes. Taken together, these results suggest that CREBZF may be novel putative marker of the spermatocyte and regulate meiosis during postnatal development of mice.
环磷酸腺苷反应元件结合蛋白张飞（CREBZF）是ATF/CREB（激活转录因子/环磷酸腺苷反应元件结合蛋白）家族的成员之一，通过与转录因子相互作用来调节众多细胞功能和细胞发育。本研究发现了CREBZF在小鼠出生后发育过程中睾丸生精小管中的表达模式。在睾丸中，CREBZF mRNA表达在其他器官中是最高的。免疫荧光分析表明，CREBZF在小鼠睾丸生精上皮的精母细胞上特异性表达，而在精原细胞和支持细胞中不表达。半定量聚合酶链反应（PCR）分析表明，在小鼠睾丸出生后发育过程中CREBZF转录水平显著升高。共聚焦成像分析表明，生精小管中CREBZF的蛋白表达在出生后第14天之前一直较低，而在出生后第21天显著增加。有趣的是，在联会复合体蛋白3（SCP3）阳性精母细胞中，只有一种类型的精母细胞特异性表达CREBZF。综上所述，这些结果表明CREBZF可能是精母细胞的新型潜在标志物，并在小鼠出生后发育过程中调节减数分裂。
