Rizzo Stefania, Petrella Francesco, Zucca Ileana, Rinaldi Elena, Barbaglia Andrea, Padelli Francesco, Baggi Fulvio, Spaggiari Lorenzo, Bellomi Massimo, Bruzzone Maria Grazia
1Department of Radiology, European Institute of Oncology, via Ripamonti 435, 20141 Milan, Italy.
2Department of Thoracic Surgery, European Institute of Oncology, Milan, Italy.
Eur Radiol Exp. 2017;1(1):6. doi: 10.1186/s41747-017-0010-9. Epub 2017 Jun 29.
Among the various stem cell populations used for cell therapy, adult mesenchymal stromal cells (MSCs) have emerged as a major new cell technology. These cells must be tracked after transplantation to monitor their migration within the body and quantify their accumulation at the target site. This study assessed whether rat bone marrow MSCs can be labelled with superparamagnetic iron oxide (SPIO) nanoparticles and perfluorocarbon (PFC) nanoemulsion formulations without altering cell viability and compared magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) results from iron-labelled and fluorine-labelled MSCs, respectively.
Of MSCs, 2 × 10 were labelled with Molday ION Rhodamine-B (MIRB) and 2 × 10 were labelled with Cell Sense. Cell viability was evaluated by trypan blue exclusion method. Labelled MSCs were divided into four samples containing increasing cell numbers (0.125 × 10, 0.25 × 10, 0.5 × 10, 1 × 10) and scanned on a 7T MRI: for MIRB-labelled cells, phantoms and cells negative control, T1, T2 and T2* maps were acquired; for Cell Sense labelled cells, phantoms and unlabelled cells, a F non-localised single-pulse MRS sequence was acquired.
In total, 86.8% and 83.6% of MIRB-labelled cells and Cell Sense-labelled cells were viable, respectively. MIRB-labelled cells were visible in all samples with different cell numbers; pellets containing 0.5 × 10 and 1 × 10 of Cell Sense-labelled cells showed a detectable F signal.
Our data support the use of both types of contrast material (SPIO and PFC) for MSCs labelling, although further efforts should be dedicated to improve the efficiency of PFC labelling.
在用于细胞治疗的各种干细胞群体中，成人间充质基质细胞（MSCs）已成为一种主要的新型细胞技术。这些细胞在移植后必须进行追踪，以监测它们在体内的迁移情况并量化它们在靶位点的聚集。本研究评估了大鼠骨髓间充质干细胞是否可以用超顺磁性氧化铁（SPIO）纳米颗粒和全氟碳（PFC）纳米乳剂制剂进行标记而不改变细胞活力，并分别比较了铁标记和氟标记的间充质干细胞的磁共振成像（MRI）和磁共振波谱（MRS）结果。
将2×10的间充质干细胞用莫尔戴离子罗丹明-B（MIRB）标记，2×10的间充质干细胞用细胞传感剂标记。通过台盼蓝排斥法评估细胞活力。将标记的间充质干细胞分为四个含有不同细胞数量增加的样本（0.125×10、0.25×10、0.5×10、1×10），并在7T MRI上进行扫描：对于MIRB标记的细胞、体模和细胞阴性对照，采集T1、T2和T2*图谱；对于细胞传感剂标记的细胞、体模和未标记的细胞，采集F非定位单脉冲MRS序列。
MIRB标记的细胞和细胞传感剂标记的细胞的存活率分别为86.8%和83.6%。在所有不同细胞数量的样本中都能看到MIRB标记的细胞；含有0.5×10和1×10细胞传感剂标记细胞的沉淀显示出可检测到的F信号。
我们的数据支持使用两种类型的造影剂（SPIO和PFC）对间充质干细胞进行标记，尽管应进一步努力提高PFC标记的效率。
