School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, 37673, Republic of Korea.
Department of Creative IT Engineering, Pohang University of Science and Technology, Pohang, 37673, Republic of Korea.
Adv Healthc Mater. 2018 Jul;7(14):e1800050. doi: 10.1002/adhm.201800050. Epub 2018 Apr 30.
Here, a new bioprinting process by combining drop-on-demand inkjet printing with a spray-coating technique, which enables the high-resolution, high-speed, and freeform fabrication of large-scale cell-laden hydrogel structures is reported. Hydrogel structures with various shapes and composed of different materials, including alginate, cellulose nanofiber, and fibrinogen, are fabricated using the inkjet-spray printing. To manufacture cell-friendly hydrogel structures with controllable stiffness, gelatine methacryloyl is saponified to stabilize jet formation and is subsequently mixed with sodium alginate to prepare blend inks. The hydrogels crosslinked from the blend inks are characterized by assessing physical properties including the microstructure and mechanical stiffness and cellular responses including the cell viability, metabolic activity, and functionality of human dermal fibroblasts within the hydrogel. Cell-laden hydrogel structures are generated on a large scale and collagen type I secretion and spreading of cells within the hydrogels are assessed. The results demonstrate that the inkjet-spray printing system will ensure the formation of a cell-laden hydrogel structure with high shape fidelity in a rapid and reliable manner. Ultimately, the proposed printing technique and the blend bioink to be used to fabricate 3D laminated large-scale tissue equivalents that potentially mimic the function of native tissues is expected.
在这里，报告了一种新的生物打印工艺，它结合按需喷墨打印和喷涂技术，能够实现大规模细胞负载水凝胶结构的高分辨率、高速和自由形态制造。使用喷墨-喷涂印刷制造了具有各种形状和由不同材料组成的水凝胶结构，包括藻酸盐、纤维素纳米纤维和纤维蛋白原。为了制造具有可控硬度的细胞友好型水凝胶结构，将明胶甲基丙烯酰基皂化以稳定射流形成，然后与海藻酸钠混合制备混合油墨。从混合油墨交联的水凝胶的物理性质，包括微结构和机械硬度以及细胞反应，包括细胞活力、代谢活性和人真皮成纤维细胞在水凝胶中的功能进行评估。大规模生成负载细胞的水凝胶结构，并评估胶原蛋白 I 的分泌和细胞在水凝胶中的扩散。结果表明，喷墨-喷涂打印系统将以快速可靠的方式确保形成具有高形状保真度的细胞负载水凝胶结构。最终，预计所提出的打印技术和混合生物墨水将用于制造 3D 层压大规模组织等效物，这些组织等效物可能模拟天然组织的功能。
