Al Lawati Haider A J, Al Mughairy Baqia, Al Lawati Iman, Suliman FakhrEldin O
Department of Chemistry, College of Science, Sultan Qaboos University, Box 36, Al-Khod 123, Oman.
Luminescence. 2018 Aug;33(5):863-870. doi: 10.1002/bio.3483. Epub 2018 Apr 30.
A novel mixing approach was utilized with a highly sensitive chemiluminescence (CL) method to determine the total phenolic content (TPC) in honey samples using an acidic potassium permanganate-formaldehyde system. The mixing approach was based on exploiting the mixing efficiency of nanodroplets generated in a microfluidic platform. Careful optimization of the instrument setup and various experimental conditions were employed to obtain excellent sensitivity. The mixing efficiency of the droplets was compared with the CL signal intensity obtained using the common serpentine chip design, with both approaches using at a total flow rate of 15 μl min ; the results showed that the nanodroplets provided 600% higher CL signal intensity at this low flow rate. Using the optimum conditions, calibration equations, limits of detection (LOD) and limits of quantification (LOQ) for gallic acid (GA), caffeic acid (CA), kaempferol (KAM), quercetin (QRC) and catechin (CAT) were obtained. The LOD ranged from 6.2 ppb for CA to 11.0 ppb for QRC. Finally, the method was applied for the determination of TPC in several local and commercial honey samples.
采用一种新型混合方法与高灵敏度化学发光（CL）法，利用酸性高锰酸钾-甲醛体系测定蜂蜜样品中的总酚含量（TPC）。该混合方法基于利用微流控平台中产生的纳米液滴的混合效率。通过仔细优化仪器设置和各种实验条件以获得出色的灵敏度。将液滴的混合效率与使用常见蛇形芯片设计获得的CL信号强度进行比较，两种方法的总流速均为15 μl/min；结果表明，在这种低流速下，纳米液滴的CL信号强度高出600%。在最佳条件下，获得了没食子酸（GA）、咖啡酸（CA）、山柰酚（KAM）、槲皮素（QRC）和儿茶素（CAT）的校准方程、检测限（LOD）和定量限（LOQ）。LOD范围从CA的6.2 ppb到QRC的11.0 ppb。最后，该方法应用于测定几种本地和市售蜂蜜样品中的TPC。
