Department of Chemistry, College of Science , Sultan Qaboos University , Box 36, Al-Khod 123 , Muscat , Oman.
Anal Chem. 2019 Aug 20;91(16):10631-10639. doi: 10.1021/acs.analchem.9b01862. Epub 2019 Jul 30.
Herein, a novel paper-based chemiluminescence (CL) device has been reported for the estimation of total phenolic content of food samples. The CL system implemented on the paper was based on a hydrogen peroxide (HO)-rhodamine b (RhoB)-cobalt metal organic framework (CoMOF) reaction. It was found that the reaction of HO with RhoB molecules, loaded into the nanopores of CoMOF (R@CoMOF), can produce an intensive CL emission. The experiments on the paper indicated that in the presence of CoMOF, the CL emission was greatly increased. In addition to this strong catalyzing effect, application of CoMOF on the paper improved the stability of the CL system for several days. As a useful analytical application for the obtained paper-based CL device (PCD), it was examined for the detection of phenolic antioxidants. It was observed that the addition of 5 μL of phenolic compounds (PC) on the paper containing the CL reagents can remarkably decrease the CL intensity. This effect was applied to design a simple analytical assay for PC. After the optimization process, the best sensitivity was obtained for gallic acid, quercetin, catechin, kaempferol, and caffeic acid with detection limits of 0.98, 1.36, 1.48, 1.81, and 2.55 ng mL, respectively. The relative standard deviations (RSD%) were also less than 5%. This study is the first report on the practical application of PCD using a nanomaterial assisted CL reaction. It is simple, portable, and low-cost and consumes a very low amount of reagents and sample solution. The device was successfully applied in the investigation of total antioxidant capacity of molasses and honey samples.
本文报道了一种用于测定食品样品总酚含量的新型纸质化学发光（CL）装置。该纸质CL 系统基于过氧化物（HO）-罗丹明 B（RhoB）-钴金属有机骨架（CoMOF）反应。研究发现，HO 与负载在 CoMOF 纳米孔中的 RhoB 分子（R@CoMOF）反应可以产生强烈的 CL 发射。实验表明，在 CoMOF 的存在下，CL 发射大大增强。除了这种强烈的催化作用外，CoMOF 在纸上的应用还使 CL 系统的稳定性提高了数天。作为获得的基于纸张的 CL 装置（PCD）的有用分析应用，它被用于检测酚类抗氧化剂。结果表明，在含有 CL 试剂的纸上加入 5 μL 的酚类化合物（PC）可以显著降低 CL 强度。这种效应被应用于设计简单的 PC 分析测定法。经过优化过程，对没食子酸、槲皮素、儿茶素、山柰酚和咖啡酸的检测限分别为 0.98、1.36、1.48、1.81 和 2.55ng mL，获得了最佳的灵敏度。相对标准偏差（RSD%）也小于 5%。这是首次报道使用纳米材料辅助 CL 反应的 PCD 的实际应用。它简单、便携、成本低，试剂和样品溶液的消耗量非常低。该装置成功应用于测定糖蜜和蜂蜜样品的总抗氧化能力。
