Poppe Jakeline Kathiele, Matte Carla Roberta, de Freitas Vitória Olave, Fernandez-Lafuente Roberto, Rodrigues Rafael C, Záchia Ayub Marco Antônio
Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9500, PO Box 15090, ZC 91501-970, Porto Alegre, RS, Brazil.
Department of Biocatalysis, ICP-CSIC, Campus UAM-CSIC, Cantoblanco, Madrid, ZC 28049, Spain.
Biotechnol Prog. 2018 Jul;34(4):952-959. doi: 10.1002/btpr.2650. Epub 2018 Jul 27.
This work describes the continuous synthesis of ethyl esters via enzymatic catalysis on a packed-bed continuous reactor, using mixtures of immobilized lipases (combi-lipases) of Candida antarctica (CALB), Thermomyces lanuginosus (TLL), and Rhizomucor miehei (RML). The influence of the addition of glass beads to the reactor bed, evaluation of the use of different solvents, and flow rate on reaction conditions was studied. All experiments were conducted using the best combination of lipases according to the fatty acid composition of the waste oil (combi-lipase composition: 40% of TLL, 35% of CALB, and 25% of RML) and soybean oil (combi-lipase composition: 22.5% of TLL, 50% of CALB, and 27.5% of RML). The best general reaction conditions were found to be using tert-butanol as solvent, and the flow rate of 0.08 mL min . The combi-lipase reactors operating at steady state for over 30 days (720 h), kept conversion yields of ∼50%, with average productivity of 1.94 g h , regardless of the type of oil in use. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:952-959, 2018.
本研究描述了在填充床连续反应器中，使用南极假丝酵母（CALB）、嗜热栖热菌（TLL）和米黑根毛霉（RML）的固定化脂肪酶混合物（复合脂肪酶）通过酶催化连续合成乙酯的过程。研究了向反应器床层添加玻璃珠、不同溶剂的使用以及流速对反应条件的影响。所有实验均根据废油的脂肪酸组成使用最佳脂肪酶组合（复合脂肪酶组成：40%的TLL、35%的CALB和25%的RML）以及大豆油（复合脂肪酶组成：22.5%的TLL、50%的CALB和27.5%的RML）进行。发现最佳的一般反应条件是使用叔丁醇作为溶剂，流速为0.08 mL/min。无论使用何种类型的油，复合脂肪酶反应器在稳态下运行超过30天（720小时），转化率保持在约50%，平均生产率为1.94 g/h。©2018美国化学工程师学会生物技术进展，34:952 - 959，2018。
