Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway.
Centre for International Health, Department of Global Public Health and Primary Care, Faculty of Medicine, University of Bergen, Bergen, Norway.
J Biomed Mater Res A. 2018 Oct;106(10):2582-2593. doi: 10.1002/jbm.a.36448.
Human exposure to titanium dioxide nanoparticles (nano-TiO ) is increasing. An internal source of nano-TiO is represented by titanium-based orthopedic and dental implants can release nanoparticles (NPs) upon abrasion. Little is known about how the size of NPs influences their interaction with cytoskeletal protein networks and the functional/homeostatic consequences that might follow at the implant-bone interface with regard to osteoblasts. We investigated the effects of size of anatase nano-TiO on SaOS-2 human osteoblast-like cells exposed to clinically relevant concentrations (0.05, 0.5, 5 mg/L) of 5 and 40 nm spherical nano-TiO . Cell viability and proliferation, adhesion, spread and migration were assessed, as well as the orientation of actin and microtubule cytoskeletal networks. The phosphorylation of focal adhesion kinase (p-FAK ) and the expression of vinculin in response to nano-TiO were also assessed. Treatment with nano-TiO disrupted the actin and microtubule cytoskeletal networks leading to morphological modifications of SaOS-2 cells. The phosphorylation of p-FAK and the expression of vinculin were also modified depending on the particle size, which affected cell adhesion. Consequently, the cell migration was significantly impaired in the 5 nm-exposed cells compared to unexposed cells. The present work shows that the orientation of cytoskeletal networks and the focal adhesion proteins and subsequently the adhesion, spread and migration of SaOS-2 cells were affected by the selected nano-TiO in a size dependent manner. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2582-2593, 2018.
人类接触二氧化钛纳米颗粒（nano-TiO）的情况越来越多。钛基骨科和牙科植入物是体内纳米 TiO 的来源，它们在磨损时会释放纳米颗粒（NPs）。目前人们对于纳米颗粒的大小如何影响它们与细胞骨架蛋白网络的相互作用以及在植入物-骨界面处对成骨细胞的功能/动态平衡的影响知之甚少。我们研究了锐钛矿纳米 TiO 的大小对暴露于临床相关浓度（0.05、0.5、5 mg/L）5nm 和 40nm 球形纳米 TiO 的 SaOS-2 人成骨样细胞的影响。评估了细胞活力和增殖、粘附、铺展和迁移，以及肌动蛋白和微管细胞骨架网络的取向。还评估了纳米 TiO 对粘着斑激酶（p-FAK）磷酸化和连接蛋白表达的影响。纳米 TiO 处理破坏了肌动蛋白和微管细胞骨架网络，导致 SaOS-2 细胞形态发生改变。p-FAK 的磷酸化和连接蛋白的表达也因颗粒大小而改变，这影响了细胞粘附。因此，与未暴露细胞相比，5nm 暴露细胞的细胞迁移明显受损。本研究表明，所选纳米 TiO 的大小依赖性影响了 SaOS-2 细胞的细胞骨架网络和粘着斑蛋白的取向，以及随后的细胞粘附、铺展和迁移。©2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A：106A：2582-2593，2018。
