Maia Keilla M, Souza Ana L P, Praxedes Erica C G, Bezerra Luana G P, Silva Andreia M, Campos Livia B, Moreira Samara S J, Apolinário Carlos A C, Souza João B F, Silva Alexandre R
Laboratory of Animal Germplasm Conservation (LCGA), Department of Animal Sciences, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, Brazil.
Laboratory of Animal Biometeorology and Environmental Biophysics, UFERSA, Mossoró, Brazil.
Biopreserv Biobank. 2018 Jun;16(3):186-190. doi: 10.1089/bio.2017.0124. Epub 2018 Apr 30.
The influence of environmental factors in a semiarid climate on characteristics of fresh and frozen/thawed sperm collected from collared peccaries () was assessed. Semen from 11 male collared peccaries was collected by electroejaculation during the peaks of the dry and rainy periods while rainfall indices, air temperatures, relative humidity levels, and wind speeds were measured. The number, motility, morphology, osmotic response, and membrane integrity of sperm in the collected ejaculates were assessed. Samples were then frozen in liquid nitrogen, thawed, and reassessed. The rainfall index of the rainy period (73.2 mm) was significantly higher than that of the dry period (13.6 mm) and the relative humidity was significantly higher during the rainy period (74.6%) than it was during the dry period (66.8%). Air temperature and wind speed did not differ between the two periods. Characteristics of sperm in the fresh samples were not affected by environmental parameters. In contrast, computerized analysis revealed that sperm in samples frozen during the rainy period exhibited better post-thaw membrane integrity (28.6 ± 6%), motility (29.5 ± 7.7%), and rapid sperm population (13.7 ± 6.2%) than did sperm in samples frozen during the dry period (23.4 ± 3% membrane integrity, 14.6 ± 4.1% motility, and 4.1 ± 1.2% rapid sperm; < 0.05). Other characteristics of the frozen/thawed sperm did not differ depending on the period in which they were collected. We demonstrated that environmental parameters did not affect the quality of fresh sperm, but could influence the freezability of sperm collected from collared peccaries raised under a semiarid climate.
评估了半干旱气候下环境因素对领西猯新鲜及冷冻/解冻精子特征的影响。在旱季和雨季高峰期，通过电刺激采精法采集了11只雄性领西猯的精液，同时测量了降雨指数、气温、相对湿度和风速。对采集的射精样本中的精子数量、活力、形态、渗透反应和膜完整性进行了评估。然后将样本在液氮中冷冻、解冻并重新评估。雨季的降雨指数（73.2毫米）显著高于旱季（13.6毫米），雨季的相对湿度（74.6%）也显著高于旱季（66.8%）。两个时期的气温和风速没有差异。新鲜样本中精子的特征不受环境参数的影响。相比之下，计算机分析显示，与旱季冷冻样本中的精子相比，雨季冷冻样本中的精子解冻后膜完整性更好（28.6±6%）、活力更高（29.5±7.7%）、快速精子群体比例更高（13.7±6.2%）（旱季冷冻样本中精子的膜完整性为23.4±3%、活力为14.6±4.1%、快速精子比例为4.1±1.2%；P<0.05）。冷冻/解冻精子的其他特征不受采集时期的影响。我们证明，环境参数不会影响新鲜精子的质量，但可能会影响在半干旱气候下饲养的领西猯所采集精子的冷冻保存能力。
