Institute of Diagnostics, School of Medicine, Shandong University, Ji'nan, Shandong, China.
Institute of Yantai, China Agricultural University, Beijing, China.
Biochem Biophys Res Commun. 2018 Jun 22;501(2):380-386. doi: 10.1016/j.bbrc.2018.04.203. Epub 2018 May 10.
Homeobox-containing 1 (HMBOX1) has been described as a transcription factor involved in the occurrence of some tumors, but its roles in ovarian cancer have never been reported. Here we aimed to investigate the roles of HMBOX1 on high-grade serous ovarian carcinoma (HGSOC). In this present study, HMBOX1 expression was decreased in HGSOC tissues and ovarian cancer cell lines (HO8910 and A2780) compared with ovarian surface epithelial tissues or normal human ovarian surface epithelial cell line (HOSEpiC). The cell proliferation of HOSEpiC was weaker than ovarian cancer cell lines. By altering the expression of HMBOX1 in A2780 and HOSEpiC, we demonstrated that HMBOX1 inhibited the cell proliferation and promoted the cell apoptosis. Furthermore, our study revealed that HMBOX1 downregulated the expression of anti-apoptotic proteins (Bcl-2, Bcl-xL), raised the expression of pro-apoptotic-regulated proteins (Bad, Bax), apoptotic executionior (Caspase3), and P53. In conclusion, HMBOX1 played important roles in occurrence of HGSOC through regulation of proliferation and apoptosis, which implied that HMBOX1 might serve as a new therapeutic target for HGSOC.
同源盒蛋白 1（HMBOX1）已被描述为一种参与某些肿瘤发生的转录因子，但它在卵巢癌中的作用从未被报道过。在这里，我们旨在研究 HMBOX1 在高级别浆液性卵巢癌（HGSOC）中的作用。在本研究中，与卵巢表面上皮组织或正常人类卵巢表面上皮细胞系（HOSEpiC）相比，HMBOX1 在 HGSOC 组织和卵巢癌细胞系（HO8910 和 A2780）中的表达降低。HOSEpiC 的细胞增殖能力弱于卵巢癌细胞系。通过改变 A2780 和 HOSEpiC 中 HMBOX1 的表达，我们证明 HMBOX1 抑制细胞增殖并促进细胞凋亡。此外，我们的研究表明 HMBOX1 下调了抗凋亡蛋白（Bcl-2、Bcl-xL）的表达，上调了促凋亡调节蛋白（Bad、Bax）、凋亡执行蛋白（Caspase3）和 P53 的表达。总之，HMBOX1 通过调节增殖和凋亡在 HGSOC 的发生中发挥重要作用，这表明 HMBOX1 可能成为 HGSOC 的新治疗靶点。
