School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
School of Medicine, Jiangsu University, Zhenjiang 212013, China.
Infect Genet Evol. 2018 Aug;62:244-252. doi: 10.1016/j.meegid.2018.04.034. Epub 2018 Apr 27.
Sapoviruses (SaVs), belonging to the genus Sapovirus of the family Caliciviridae, were known as the enteric pathogen causing acute gastroenteritis. SaVs have been detected in humans and several animals including pigs and some porcine SaVs showed close sequence relationship with human strains suggesting the possibility of interspecies transmission. Here, we sequenced the genomes of two porcine SaVs (with strain names of p38 and SH1703) using the metagenomic analyses and traditional RT-PCR methods. Phylogenetic trees were constructed based on the complete genome, the full-length VP 1 nucleotide and amino acid sequences to group those two strains. The two porcine SaV strains, p38 and SH1703, detected in this study, were classified as genogroup III and genogroup VII, respectively. These two strains showed similar genomic organization with that of other SaVs. We firstly divided SaVs into 51 genotypes within 19 genogroups. Our data are helpful for genetic characterization and classification of newly detected SaVs worldwide.
肠道病毒（SaVs）属于杯状病毒科的肠道病毒属，是已知引起急性胃肠炎的肠道病原体。SaVs 已在人类和包括猪在内的几种动物中被检测到，一些猪源 SaVs 与人类株具有密切的序列关系，提示存在种间传播的可能性。在这里，我们使用宏基因组分析和传统 RT-PCR 方法对两种猪源 SaVs（分别命名为 p38 和 SH1703）进行了基因组测序。基于完整基因组、全长 VP1 核苷酸和氨基酸序列构建了系统进化树，对这两株进行了分组。本研究中检测到的两株猪源 SaV 株 p38 和 SH1703 分别属于基因 III 组和基因 VII 组。这两株与其他 SaVs 具有相似的基因组组织。我们首次将 SaVs 在 19 个基因群中分为 51 个基因型。我们的数据有助于对全球新检测到的 SaVs 进行遗传特征和分类。
