Biosignal Research Center, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan; University of Belgrade, Faculty of Chemistry, P.O. Box 51, Studentski trg 12, 11158 Belgrade, Serbia.
Osaka City Institute of Public Health and Environmental Sciences, 8-34 Tojocho, Tennoji-ku, Osaka 543-0026, Japan.
Sci Total Environ. 2018 Sep 15;636:355-359. doi: 10.1016/j.scitotenv.2018.04.243. Epub 2018 Apr 27.
We investigated the capability of microorganisms isolated from environments polluted with perfluoroalkyl acids (PFAAs) to conduct biotransformation of these emerging pollutants. Two different microbial consortia (chemoorganoheterotrophic bacteria and total yeast and molds) were isolated from two river sediments in Saitama and Osaka, Japan, known for long term pollution with perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA). The microbial consortia were incubated in the presence of added PFOS and PFOA, and decreases in concentrations of these compounds were between 46-69% and 16-36%, respectively. Decreases in concentrations were, in part, due to sorption on biomass, but defluorinated PFOS and PFOA products were not detected. However, untargeted analysis suggested the presence of several metabolites found only in samples from consortia with PFOS and PFOA but not in the control samples. Molecular formula candidates were narrowed down to two options, CHOF and CHO. It was assumed that these formulas were associated with unsaturated monofluorinated fatty acids and hydrocarbons with multiple unsaturated bonds or ring structures.
我们研究了从受全氟烷基酸 (PFAAs) 污染的环境中分离出的微生物将这些新兴污染物进行生物转化的能力。从日本埼玉和大阪的两条河流沉积物中分离出了两种不同的微生物群落（化能有机异养菌和总酵母和霉菌），这些河流因长期受到全氟辛烷磺酸 (PFOS) 和全氟辛酸 (PFOA) 的污染而闻名。在添加 PFOS 和 PFOA 的情况下培养微生物群落，这些化合物的浓度分别下降了 46-69%和 16-36%。浓度的下降部分归因于生物量的吸附，但未检测到脱氟 PFOS 和 PFOA 产物。然而，非靶向分析表明，仅在含有 PFOS 和 PFOA 的群落样本中存在几种代谢产物，而在对照样本中则不存在。分子公式候选者被缩小到两个选项，CHOF 和 CHO。据推测，这些公式与不饱和单氟脂肪酸以及具有多个不饱和键或环结构的烃有关。
