Metzger Axel, Anslyn Eric V
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, TX 78712 (USA), Fax: (+1) 512-471-8696.
Angew Chem Int Ed Engl. 1998 Mar 16;37(5):649-652. doi: 10.1002/(SICI)1521-3773(19980316)37:5<649::AID-ANIE649>3.0.CO;2-H.
Competitive binding like that in immunoassays is the principle behind the chemosensor based on receptor 1, which was used to measure the concentration of citrate (2) in a series of common beverages. Citrate displaces the fluorescent colorimetric probe 5-carboxylfluorescein (3) from 1, and the process can be monitored by UV or fluorescence spectroscopy.
免疫分析中的竞争性结合是基于受体1的化学传感器的原理,该传感器用于测量一系列常见饮料中柠檬酸盐(2)的浓度。柠檬酸盐将荧光比色探针5-羧基荧光素(3)从1中置换出来,这一过程可以通过紫外光谱或荧光光谱进行监测。