Detection of Tick-Borne Pathogens in Lambs Undergoing Prophylactic Treatment Against Ticks on Two Swedish Farms.

Tick-borne pathogens (TBPs), especially , cause disease in grazing livestock. Tick prophylaxis is, therefore, a routine practice in sheep flocks in Sweden, especially in central, southern, and coastal areas of the country where ixodid ticks ( and ) are present. In the present study, the status of infection by and other TBPs in lambs treated with tick prophylaxis has been assessed serologically and with polymerase chain reaction (PCR). Blood samples ( = 78) from lambs ( = 20) subjected to regular tick prophylactic treatment (flumethrin, Bayticol) at two sites in different regions in Sweden (Östergötland, Gotland) were collected on four occasions from May until July 2013. The severity of clinical signs in infected animals is known to differ between these two regions. In total, 20% of blood samples were PCR-positive for . Serological analyses showed that 33% of all collected samples were positive for , while 2.5% were positive for s.l. and 13% for tick-borne encephalitis virus (TBEV). Percentages of lambs positive were 75 and 45% for antibodies and DNA, respectively, while 10 and 45% were serologically positive for s.l. and TBEV, respectively. Sequencing of partial 16S rRNA genes from PCR positive samples revealed presence of in all animals in Östergötland, while sequences consistent with as well as and were found on the island of Gotland. This is the first report of the occurrence of the latter two species in Sweden.