黄酮卡瓦因B与柔红霉素联合通过调节核因子κB诱导人髓系白血病细胞凋亡。
The Combination of Flavokawain B and Daunorubicin Induces Apoptosis in Human Myeloid Leukemic Cells by Modifying NF-κB.
作者信息
Lee Jae Jin, Koh Kyung-Nam, Park Chan-Jeoung, Jang Seongsoo, Im Ho Joon, Kim Nayoung
机构信息
Asan Institute for Life Sciences and Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
出版信息
Anticancer Res. 2018 May;38(5):2771-2778. doi: 10.21873/anticanres.12520.
BACKGROUND/AIM: Flavokawain B (FKB), is a natural chalcone isolated from kava root that induces apoptosis in cancer cells. Herein we investigated the effects of combination of FKB and daunorubicin (DNR) on human leukemic cells.
MATERIALS AND METHODS
Cell viability and death were assessed by the MTS assay and flow cytometry. NK-κB was detected by western blotting.
RESULTS
FKB alone and in combination with DNR reduced the viable cell numbers of four leukemic cell lines. FKB itself induced apoptosis of an acute myeloid cell line, HL-60. Because the additive effect of DNR and FKB was most obvious in HL-60 cells, subsequent experiments were performed with HL-60 cells. Combined treatment of the two compounds increased NF-κB activation at 12 h.
CONCLUSION
A combination treatment of DNR and FKB may improve the anticancer effects of DNR in DNR-resistant acute myeloid leukemia.
背景/目的：黄樟素B（FKB）是从卡瓦根中分离出的一种天然查尔酮，可诱导癌细胞凋亡。在此，我们研究了FKB与柔红霉素（DNR）联合使用对人白血病细胞的影响。
材料与方法
通过MTS法和流式细胞术评估细胞活力和死亡情况。通过蛋白质免疫印迹法检测核因子κB（NK-κB）。
结果
单独使用FKB以及FKB与DNR联合使用均降低了四种白血病细胞系的活细胞数量。FKB自身可诱导急性髓性细胞系HL-60凋亡。由于DNR和FKB的相加效应在HL-60细胞中最为明显，因此随后的实验在HL-60细胞中进行。两种化合物联合处理在12小时时增加了NF-κB的激活。
结论
DNR与FKB联合治疗可能会提高DNR对耐药急性髓性白血病的抗癌效果。
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