López-Muñoz Gerardo A, Estévez M-Carmen, Vázquez-García Marc, Berenguel-Alonso Miguel, Alonso-Chamarro Julián, Homs-Corbera Antoni, Lechuga Laura M
Nanobiosensors and Bioanalytical Applications Group (NanoB2A), Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, Bellaterra, 08193, Barcelona, Spain.
CIBER-BBN Networking Center in Bioengineering, Biomaterials and Nanomedicine, Barcelona, Spain.
J Biophotonics. 2018 Aug;11(8):e201800043. doi: 10.1002/jbio.201800043. Epub 2018 May 24.
Ultrasmooth gold/silver/gold trilayer nanostructured plasmonic sensors were obtained using commercial Blu-ray optical discs as nanoslits-based flexible polymer substrates. A thin gold film was used as an adhesion and nucleation layer to improve the chemical stability and reduce the surface roughness of the overlying silver film, without increasing ohmic plasmon losses. The structures were physically and optically characterized and compared with nanostructures of single gold layer. Ultrasmooth and chemically stable trilayer nanostructures with a surface roughness <0.5 nm were obtained following a simple and reproducible fabrication process. They showed a figure of merit (FOM) value up to 69.2 RIU which is significantly higher (more than 95%) than the gold monolayer counterpart. Their potential for biosensing was demonstrated by employing the trilayer sensor for the direct and refractometric (label-free) detection of C-reactive protein (CRP) biomarker in undiluted urine achieving a Limit of Detection (LOD) in the pM order.
利用商用蓝光光盘作为基于纳米狭缝的柔性聚合物基板，获得了超光滑的金/银/金三层纳米结构等离子体传感器。使用薄金膜作为粘附和成核层，以提高化学稳定性并降低上覆银膜的表面粗糙度，同时不增加欧姆等离子体损耗。对这些结构进行了物理和光学表征，并与单层金纳米结构进行了比较。通过简单且可重复的制造工艺，获得了表面粗糙度<0.5 nm的超光滑且化学稳定的三层纳米结构。它们的品质因数（FOM）值高达69.2 RIU，比单层金对应物显著更高（超过95%）。通过将三层传感器用于直接和折射（无标记）检测未稀释尿液中的C反应蛋白（CRP）生物标志物，实现了皮摩尔级的检测限，证明了其在生物传感方面的潜力。
