Department of Mechanical Engineering, National Chung-Hsing University, Taichung, Taiwan.
Int J Nanomedicine. 2012;7:133-40. doi: 10.2147/IJN.S27225. Epub 2012 Jan 6.
This study integrates the techniques of nanoelectroforming, hot-embossing, and electrochemical deposition to develop a disposable, low-cost, and high sensitivity nanostructure biosensor. A modified anodic aluminum oxide barrier-layer surface was used as the template for thin nickel film deposition. After etching the anodic aluminum oxide template off, a three-dimensional mold of the concave nanostructure array was created. The fabricated three-dimensional nickel mold was further used for replica molding of a nanostructure polycarbonate substrate by hot-embossing. A thin gold film was then sputtered onto the polycarbonate substrate to form the electrode, followed by deposition of an orderly and uniform gold nanoparticle layer on the three-dimensional gold electrode using electrochemical deposition. Finally, silver nanoparticles were deposited on the uniformly deposited gold nanoparticles to enhance the conductivity of the sensor. Electrochemical impedance spectroscopy analysis was then used to detect the concentration of the target element. The sensitivity of the proposed scheme on the detection of the dust mite antigen, Der p2, reached 0.1 pg/mL.
本研究整合了纳米电铸、热压印和电化学沉积技术，开发出一种一次性、低成本、高灵敏度的纳米结构生物传感器。采用改性的阳极氧化铝阻挡层表面作为沉积薄镍膜的模板。去除阳极氧化铝模板后，形成了具有凹型纳米结构阵列的三维模具。所制备的三维镍模具进一步通过热压印用于复制成型纳米结构聚碳酸酯基底。然后将一层薄金膜溅射在聚碳酸酯基底上形成电极，接着通过电化学沉积在三维金电极上沉积有序且均匀的金纳米粒子层。最后，在均匀沉积的金纳米粒子上沉积银纳米粒子以增强传感器的导电性。然后使用电化学阻抗谱分析来检测目标元素的浓度。所提出方案在检测尘螨抗原 Der p2 时的灵敏度达到 0.1 pg/mL。
