Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, SAR, China.
Research and Development Centre for Natural Health Products, HKBU Shenzhen Research Institute and Continuing Education, Shenzhen, China.
BMC Complement Altern Med. 2018 May 2;18(1):141. doi: 10.1186/s12906-018-2205-x.
Herba Siegesbeckiae (HS, Xixiancao in Chinese) is a commonly used traditional Chinese medicinal herb for soothing joints. In ancient materia medica books, HS is recorded to be the aerial part of Siegesbeckia pubescens Makino (SP) which is also the only origin of HS in the 1963 edition of the Chinese Pharmacopeia (ChP). The aerial parts of Siegesbeckia orientalis L. (SO) and Siegesbeckia glabrescens Makino (SG) have been included as two additional origins for HS in each edition of ChP since 1977. However, chemical and pharmacological comparisons among these three species have not been conducted.
An HPLC with diode array detector (HPLC-DAD) method combined with similarity analysis, hierarchical cluster analysis (HCA) and principal component analysis (PCA) was developed for comparing the fingerprint chromatograms of the three species. The inhibitory effects of the three species on NO production and IL-6 secretion in LPS-stimulated RAW264.7 macrophages were compared.
Fingerprint chromatograms of the three species showed different profiles, but had 13 common peaks. Results from HCA and PCA of the common peaks demonstrated that all 14 herbal samples of the three species tended to be grouped and separated species dependently. The extents of inhibition on NO production and IL-6 secretion of the three species were different, with SG being the most and SP the least potent.
Both chemical profiles and inflammatory mediator-inhibitory effects of the three species were different. These findings provide a chemical and pharmacological basis for determining whether the three species can all serve as the origins of HS.
夏枯草（HS，中文名为细仙草）是一种常用的传统中药，用于舒缓关节。在古代本草书籍中，HS 被记录为飞扬草（SP）的地上部分，这也是 1963 年版《中国药典》（ChP）中 HS 的唯一来源。自 1977 年以来，每版《中国药典》都将三叶委陵菜（SO）和毛夏枯草（SG）的地上部分收录为 HS 的另外两个来源。然而，这三种植物的化学成分和药理作用尚未进行比较。
建立了一种高效液相色谱法（HPLC）与二极管阵列检测器（HPLC-DAD）结合相似度分析、层次聚类分析（HCA）和主成分分析（PCA）的方法，用于比较三种植物的指纹图谱。比较了三种植物对 LPS 刺激的 RAW264.7 巨噬细胞中 NO 产生和 IL-6 分泌的抑制作用。
三种植物的指纹图谱显示出不同的图谱，但有 13 个共有峰。共有峰的 HCA 和 PCA 结果表明，三种植物的 14 个草药样本都倾向于分组，并且可以根据物种进行分离。三种植物对 NO 产生和 IL-6 分泌的抑制程度不同，SG 的抑制作用最强，SP 的抑制作用最弱。
三种植物的化学成分和抗炎介质抑制作用均存在差异。这些发现为确定这三种植物是否都可以作为 HS 的来源提供了化学和药理学依据。
