Feng Siwen, Xu Gonghao, Fu Yuchen, Ding Qi, Shi Yuanyuan
School of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, China.
Shenzhen Research Institute, Beijing University of Chinese Medicine, Shenzhen 518118, China.
ACS Omega. 2023 Mar 9;8(11):10202-10213. doi: 10.1021/acsomega.2c07366. eCollection 2023 Mar 21.
Asthma is a chronic respiratory disease. Bergamot essential oil (BEO) is extracted from the bergamot peel, which is widely used as a medicinal and food plant in China. Modern pharmacological studies have confirmed that BEO has anti-inflammatory properties, suggesting potential in treating asthma. First, the main active ingredients of BEO were detected and analyzed by gas chromatography-mass spectrometry (GC-MS). Network pharmacology methods were used to explore the possible core targets and main pathways of BEO in asthma treatment. Then ovalbumin (OVA)-induced and lipopolysaccharide (LPS)-induced models were established to investigate the antiasthmatic effects of BEO. BEO showed a good antiasthmatic effect by improving lung inflammation and inhibiting collagen deposition. Then, enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qPCR) were used to explore the possible mechanism of BEO in asthma treatment. Furthermore, experimental verification showed that BEO could suppress the release of inflammatory factors and inhibit the activation of MAPK and JAK-STAT signaling pathways. This study demonstrated the anti-inflammatory effects of BEO against asthma. Moreover, it supplies a theoretical basis for the clinical application of BEO.
哮喘是一种慢性呼吸道疾病。佛手柑精油（BEO）从佛手柑果皮中提取，在中国被广泛用作药用和食用植物。现代药理学研究证实，BEO具有抗炎特性，提示其在治疗哮喘方面具有潜力。首先，采用气相色谱-质谱联用（GC-MS）法检测和分析了BEO的主要活性成分。运用网络药理学方法探究BEO在哮喘治疗中的可能核心靶点和主要通路。然后建立卵清蛋白（OVA）诱导和脂多糖（LPS）诱导的模型，以研究BEO的抗哮喘作用。BEO通过改善肺部炎症和抑制胶原沉积显示出良好的抗哮喘效果。接着，采用酶联免疫吸附测定（ELISA）和定量实时聚合酶链反应（qPCR）探究BEO在哮喘治疗中的可能机制。此外，实验验证表明，BEO可抑制炎症因子的释放，并抑制MAPK和JAK-STAT信号通路的激活。本研究证明了BEO对哮喘的抗炎作用。此外，它为BEO的临床应用提供了理论依据。
