George C X, Samuel C E
Department of Biological Sciences, University of California, Santa Barbara 93106.
Virology. 1988 Oct;166(2):573-82. doi: 10.1016/0042-6822(88)90528-4.
The effect of interferon on the expression of the reovirus serotype 1 Lang strain S3 gene was examined in simian COS cells transfected with the expression vector pSVS3 containing the S3 gene under the control of the SV40 late promoter. When COS cells were treated with type I interferon-alpha 24 hr after transfection, the synthesis of the reovirus S3-encoded sigma NS polypeptide was inhibited about 10-fold as compared to that in untreated control cells. By contrast, under the same conditions, neither the plasmid DNA copy number nor the S3 gene mRNA levels were significantly affected by interferon treatment. Type II interferon-gamma, unlike the type I interferons-alpha, did not affect the rate of synthesis of polypeptide sigma NS in pSVS3-transfected cells.
在转染了含有受SV40晚期启动子控制的S3基因的表达载体pSVS3的猴COS细胞中，研究了干扰素对呼肠孤病毒1型朗株S3基因表达的影响。转染后24小时用I型干扰素-α处理COS细胞时，与未处理的对照细胞相比，呼肠孤病毒S3编码的σNS多肽的合成受到约10倍的抑制。相比之下，在相同条件下，干扰素处理对质粒DNA拷贝数和S3基因mRNA水平均无显著影响。与I型干扰素-α不同，II型干扰素-γ不影响pSVS3转染细胞中多肽σNS的合成速率。
