呼肠孤病毒衣壳多肽σ3在共转染的COS细胞中对氯霉素乙酰转移酶mRNA翻译的刺激作用。
Stimulation of chloramphenicol acetyltransferase mRNA translation by reovirus capsid polypeptide sigma 3 in cotransfected COS cells.
作者信息
Giantini M, Shatkin A J
机构信息
Center for Advanced Biotechnology and Medicine, Piscataway, New Jersey 08855-0759.
出版信息
J Virol. 1989 Jun;63(6):2415-21. doi: 10.1128/JVI.63.6.2415-2421.1989.
Abstract
The mammalian reovirus S4 gene has been implicated in the serotype-dependent inhibition of host cell protein synthesis during viral replication in mouse L cells. To examine the effect(s) of this gene on transcription or translation or both, a DNA copy of the serotype 3 S4 gene was inserted into a eucaryotic expression vector. Cotransfection of COS cells with plasmids containing S4 and the reporter gene, chloramphenicol acetyltransferase (CAT), resulted in a marked stimulation of CAT expression, predominantly at the level of translation. The significance of these findings is discussed in relation to the double-stranded-RNA-binding activity of the S4 gene product, polypeptide sigma 3.
摘要
哺乳动物呼肠孤病毒S4基因与病毒在小鼠L细胞中复制期间宿主细胞蛋白质合成的血清型依赖性抑制有关。为了研究该基因对转录或翻译或两者的影响，将3型S4基因的DNA拷贝插入真核表达载体。将含有S4的质粒与报告基因氯霉素乙酰转移酶（CAT）共转染COS细胞，导致CAT表达显著增加，主要在翻译水平。结合S4基因产物σ3多肽的双链RNA结合活性讨论了这些发现的意义。
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