College of Bioengineering, Henan University of Technology, Zhengzhou, 450001, Henan, China.
College of Food Science and Technology, Henan University of Technology, Zhengzhou, 450001, Henan, China.
Environ Sci Pollut Res Int. 2018 Jul;25(19):18990-19000. doi: 10.1007/s11356-018-2022-2. Epub 2018 May 2.
Simulation experiments were conducted using sediments collected from the Taihu Lake to determine the combined effects of submerged macrophytes Ceratophyllum demersum and phosphate-solubilizing bacteria (PSB) strain XMT-5 (Rhizobium sp.) on phosphorus (P) concentrations in overlying waters and sediments. After 30 days of experimental incubation, the total phosphorus (TP) and dissolved total phosphorus (DTP) concentrations of the overlying water subjected to AMB and AHMB treatments (both with the combined effects of PSB cells and submerged macrophytes) were generally lower than those of the AM (with individual effects of inoculated C. demersum) and AB (with individual effects of a smaller amount of inoculated PSB cells) control treatments but higher than that of the A (with no effects of inoculated PSB cells or C. demersum) and AHB (with individual effects of a larger amount of inoculated PSB) control treatments. The TP contents of the sediment in the AMB and AHMB treatments were significantly lower than those of the other control treatments. The TP contents of the C. demersum cocultured with the PSB strain XMT-5 cells in the AMB and AHMB treatments were all significantly higher than that of the AM treatment, indicating the enhancement of P uptake by submerged plants inoculated with PSB. The bacterial diversity structures of the rhizosphere sediment subjected to different treatments were also analyzed by the high-throughput sequencing method. According to the ACE and Chao 1 indices, the bacterial diversity in the AMB and AHMB treatments were the highest. Although many sources contributed to the decrease in the nutrient loads of the lake sediment, harvesting macrophytes inoculated with PSB cells prior to their senescence might constitute a significant in-lake measure for reducing internal P load.
采用太湖沉积物进行模拟实验，研究了沉水植物金鱼藻和溶磷菌（PSB）菌株 XMT-5（根瘤菌属）联合作用对水体和沉积物中磷（P）浓度的综合影响。经过 30 天的实验培养，AMB 和 AHMB 处理（均有 PSB 细胞和沉水植物的联合作用）的上覆水总磷（TP）和溶解总磷（DTP）浓度通常低于 AM（接种金鱼藻的单独作用）和 AB（接种少量 PSB 细胞的单独作用）对照处理，但高于 A（无接种 PSB 细胞或金鱼藻的单独作用）和 AHB（接种大量 PSB 细胞的单独作用）对照处理。AMB 和 AHMB 处理的沉积物 TP 含量明显低于其他对照处理。AMB 和 AHMB 处理中与 PSB 菌株 XMT-5 共培养的金鱼藻的 TP 含量均显著高于 AM 处理，表明接种 PSB 的沉水植物对 P 的吸收增强。还通过高通量测序方法分析了不同处理下根际沉积物的细菌多样性结构。根据 ACE 和 Chao1 指数，AMB 和 AHMB 处理的细菌多样性最高。尽管许多因素导致了湖底沉积物营养负荷的降低，但在沉水植物衰老前收获接种 PSB 细胞的沉水植物可能是减少湖泊内部 P 负荷的重要湖泊措施。
