Departments of Biophysics and Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas.
Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas.
Protein Sci. 2018 Aug;27(8):1450-1463. doi: 10.1002/pro.3438. Epub 2018 Jun 13.
Viruses are the most abundant life form and infect practically all organisms. Consequently, these obligate parasites are a major cause of human suffering and economic loss. Rossmann-like fold is the most populated fold among α/β-folds in the Protein Data Bank and proteins containing Rossmann-like fold constitute 22% of all known proteins 3D structures. Thus, analysis of viral proteins containing Rossmann-like domains could provide an understanding of viral biology and evolution as well as could propose possible targets for antiviral therapy. We provide functional and evolutionary analysis of viral proteins containing a Rossmann-like fold found in the evolutionary classification of protein domains (ECOD) database developed in our lab. We identified 81 protein families of bacterial, archeal, and eukaryotic viruses in light of their evolution-based ECOD classification and Pfam taxonomy. We defined their functional significance using enzymatic EC number assignments as well as domain-level family annotations.
病毒是最丰富的生命形式，几乎感染所有生物体。因此，这些专性寄生虫是人类痛苦和经济损失的主要原因。Rossmann 样折叠是蛋白质数据库中 α/β 折叠中最常见的折叠，含有 Rossmann 样折叠的蛋白质构成所有已知蛋白质 3D 结构的 22%。因此，对含有 Rossmann 样结构域的病毒蛋白进行分析，可以了解病毒的生物学和进化，并为抗病毒治疗提出可能的靶点。我们提供了实验室开发的蛋白质结构域进化分类（ECOD）数据库中发现的含有 Rossmann 样折叠的病毒蛋白的功能和进化分析。根据基于进化的 ECOD 分类和 Pfam 分类，我们确定了 81 种细菌、古菌和真核病毒的蛋白质家族。我们使用酶 EC 编号分配以及域级家族注释来定义它们的功能意义。
