Beijing Laboratory of Biomedical Materials, College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, P. R. China.
Macromol Rapid Commun. 2018 Oct;39(20):e1800075. doi: 10.1002/marc.201800075. Epub 2018 May 2.
Rapidly separating genepin-crosslinked gelatin (RS-GC) microneedles (MNs) mounted on the polyvinyl alcohol (PVA)-coated polylactic acid (PLA) MNs (RS-PGC-MNs) are fabricated, in which GC-MNs deliver insulin within the skin and the PLA supporting array is easily separated by the dissolution of the PVA layer. The release of insulin is controlled by utilizing the virtue of genipin as a crosslinking agent for producing biocompatible GC-MNs. The degree of crosslinking enhances the mechanical strength as well as humidity resistance. The in vitro and in vivo insulin release tests show significant changes in the release rates in the RS-PGC-MNs with different crosslinking degree. The hypoglycemic effect in diabetic mice demonstrate that the higher crosslinking GC-MNs result in characteristic controlled insulin release compared with other treatments and prolonged effectiveness of the RS-PGC-MNs. The proposed RS-PGC-MNs is a promising device for effective use as a noninvasive and painless controlled insulin delivery system.
快速分离基因交联明胶（RS-GC）微针（MNs）固定在聚乙烯醇（PVA）涂覆的聚乳酸（PLA）MNs（RS-PGC-MNs）上，其中 GC-MNs 将胰岛素递送至皮肤内，而 PLA 支撑阵列则通过 PVA 层的溶解而轻易分离。胰岛素的释放通过利用京尼平作为交联剂的优点来控制，以产生生物相容性的 GC-MNs。交联程度增强了机械强度和耐湿性。体外和体内胰岛素释放测试显示，不同交联程度的 RS-PGC-MNs 的释放速率有明显变化。糖尿病小鼠的降血糖效果表明，与其他治疗方法相比，较高交联的 GC-MNs 导致特征性的控制胰岛素释放，并且 RS-PGC-MNs 的效果更持久。所提出的 RS-PGC-MNs 是一种很有前途的设备，可有效用作非侵入性和无痛的控制胰岛素输送系统。
