a Faculty of Natural Sciences and Life, Nursing Department , University of Abdelhamid Ibn Badis of Mostaganem , Mostaganem , Algeria.
b Laboratoire de Recherche «Santé et Production Animale» , Ecole Nationale Supérieure Vétérinaire d'El-Harrach , Algiers , Algeria.
Nat Prod Res. 2019 Sep;33(18):2647-2654. doi: 10.1080/14786419.2018.1466124. Epub 2018 May 4.
The aim was to evaluate the susceptibility of producing to Slovakian essential oil (TVEO) alone and in combination with cefotaxime (CTX). TVEO composition was determined by gas chromatograph-mass spectrometer (GC/MS). Susceptibility to 21 antibiotics was determined by disc diffusion assay. Genes characterization for resistance to β-lactams was accomplished by polymerase chain reaction (PCR). The antibacterial activity was investigated by standard methods. The synergistic interaction was determined by checkerboard test. Thymol (34.5%), p-cymene (22.27%) and linalool (5.35%) were the major components present in the TVEO. The identified strains were multi-drug resistant (MDR). TVEO showed high activity against all MDR strains, including producing isolates, with inhibition zones and MIC values in the range of 24-40 mm/10μL and 2.87-11.5 μg/mL, respectively. TVEO in combination with CTX showed a synergistic action against producing (FICI 0.28) and an additive effect ESBL producing (FICI 0.987).
目的是评估斯洛伐克香精油（TVEO）单独使用和与头孢噻肟（CTX）联合使用对产的敏感性。通过气相色谱-质谱联用仪（GC/MS）确定 TVEO 的组成。通过圆盘扩散法测定对 21 种抗生素的敏感性。通过聚合酶链反应（PCR）完成对β-内酰胺类药物耐药基因的特征分析。采用标准方法研究了抗菌活性。通过棋盘试验确定协同作用。百里香酚（34.5%）、对伞花烃（22.27%）和芳樟醇（5.35%）是 TVEO 中的主要成分。鉴定出的菌株为多药耐药（MDR）。TVEO 对所有 MDR 菌株均显示出高活性，包括产分离株，其抑制带和 MIC 值范围分别为 24-40 毫米/10μL 和 2.87-11.5μg/mL。TVEO 与 CTX 联合使用对产（FICI 0.28）和产 ESBL（FICI 0.987）具有协同作用。
