Kim J S, Jang H J, Jeong E K, Kim S S, Kim Y M, Lee J H, Oh M Y, Lee D K, Yelithao K, Kwan H C, Nho C W, Eom D W
Department of Anesthesia and Pain Medicine, Ulsan University College of Medicine, Gangneung Asan Hospital, Gangneung, South Korea.
Department of Surgery, Ulsan University College of Medicine, Gangneung Asan Hospital, Gangneung, South Korea.
Transplant Proc. 2018 May;50(4):1160-1166. doi: 10.1016/j.transproceed.2018.02.048.
Crepidiastrum denticulatum (CD) is a well-known, traditionally consumed vegetable in Korea, which was recently reported to contain bioactive compounds with detoxification and antioxidant properties. Ischemia-reperfusion injury (IRI) is a major problem after renal transplantation. Furthermore, inflammatory responses to IRI exacerbate the resultant renal injury. In the present study, we investigated whether CD extract exhibits renoprotective effects against IR-induced acute kidney injury in mice.
Renal IRI was induced in male C57BL/6 mice by bilateral renal pedicle occlusion for 30 minutes followed by reperfusion for 48 hours. CD extract (75 mg/kg) was administered orally 5 days before IRI.
Treatment with CD extract significantly decreased blood urea nitrogen and serum creatinine levels as well as kidney tubular injury. CD also prevented IRI-induced renal glutathione depletion and increased malondialdehyde levels. Western blotting and reverse transcriptase polymerase chain reaction indicated that CD extract significantly attenuates inducible nitric oxide synthase and toll-like receptor 2/4 protein levels 48 h after IRI. The expression of tumor necrosis factor-α and interleukin-1β was significantly decreased in the CD extract treatment group.
CD extract improved acute renal IRI through its antioxidant and anti-inflammatory effects. These findings suggest that CD extract is a potential therapeutic agent for acute ischemia-induced renal damage.
齿缘苦荬菜（CD）是韩国一种广为人知的传统食用蔬菜，最近有报道称其含有具有解毒和抗氧化特性的生物活性化合物。缺血再灌注损伤（IRI）是肾移植后的一个主要问题。此外，对IRI的炎症反应会加重由此导致的肾损伤。在本研究中，我们调查了CD提取物是否对小鼠IR诱导的急性肾损伤具有肾保护作用。
通过双侧肾蒂阻断30分钟，然后再灌注48小时，在雄性C57BL/6小鼠中诱导肾IRI。在IRI前5天口服给予CD提取物（75mg/kg）。
CD提取物治疗显著降低了血尿素氮和血清肌酐水平以及肾小管损伤。CD还可防止IRI诱导的肾谷胱甘肽耗竭并增加丙二醛水平。蛋白质印迹法和逆转录聚合酶链反应表明，CD提取物在IRI后48小时显著降低诱导型一氧化氮合酶和Toll样受体2/4蛋白水平。CD提取物治疗组肿瘤坏死因子-α和白细胞介素-1β的表达显著降低。
CD提取物通过其抗氧化和抗炎作用改善急性肾IRI。这些发现表明，CD提取物是急性缺血性肾损伤的潜在治疗药物。
