• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

石榴皮苷通过TGF-β介导的氧化应激和细胞周期减轻氧糖剥夺/复氧触发的神经母细胞瘤细胞SH-SY5Y损伤。

Punicalin Alleviates OGD/R-Triggered Cell Injury via TGF--Mediated Oxidative Stress and Cell Cycle in Neuroblastoma Cells SH-SY5Y.

作者信息

Yang Tiansong, Wang Qingyong, Qu Yuanyuan, Liu Yan, Feng Chuwen, Wang Yulin, Sun Weibo, Sun Zhongren, Zhu Yulan

机构信息

First affiliated hospital, Heilongjiang University of Chinese Medicine, Harbin, China.

Heilongjiang University of Chinese Medicine, Harbin, China.

出版信息

Evid Based Complement Alternat Med. 2021 Feb 12;2021:6671282. doi: 10.1155/2021/6671282. eCollection 2021.

DOI:10.1155/2021/6671282
PMID:33628309
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7895578/
Abstract

PURPOSE

The research aimed to identify the active component from L. to alleviate ischemia/reperfusion injury and clarify the underlying mechanism of the active component alleviating ischemia/reperfusion injury.

MATERIALS AND METHODS

The SH-SY5Y cell model of oxygen-glucose deprivation/reoxygenation (OGD/R) was established to simulate the ischemia/reperfusion injury. According to the strategy of bioassay-guided isolation, the active component of punicalin from L. was identified. Flow cytometry and Western blotting were employed to evaluate the effects of OGD/R and/or punicalin on cell cycle arrest. Immunofluorescence assay was applied to assess the nucleus translocation. The relative content of ROS and GSH and the enzyme activities of CAT and SOD were examined using ELISA.

RESULTS

The data of bioassay-guided isolation showed that punicalin from L. could alleviate OGD/R-induced cell injury in SH-SY5Y cells. Flow cytometry analysis and Western blotting for probing the expression of CDK1, p-CDK1, cyclin B1, and p21 revealed that punicalin could relieve OGD/R-induced cell cycle G0/G1 arrest. Additionally, immunofluorescence assay and Western blotting for probing the expression of TGF- and p-Smad2/p-Smad3 showed that punicalin could relieve the OGD/R-induced TGF-/Smad pathway. Furthermore, the TGF-/Smad pathway inhibitor of LY2157299 was employed to confirm that the TGF-/Smad pathway is crucial to the effect of punicalin. At last, it was indicated that punicalin could relieve OGD/R-induced oxidative stress.

CONCLUSION

Punicalin, an active component from L., was identified as a protective agent to alleviate the OGD/R-induced cell injury, which could exert the protective effect via TGF-/Smad pathway-regulated oxidative stress and cell cycle arrest in SH-SY5Y cells.

摘要

目的

本研究旨在从[植物名称]中鉴定出活性成分，以减轻缺血/再灌注损伤，并阐明该活性成分减轻缺血/再灌注损伤的潜在机制。

材料与方法

建立氧糖剥夺/复氧（OGD/R）的SH-SY5Y细胞模型以模拟缺血/再灌注损伤。根据生物活性导向分离策略，鉴定出[植物名称]中石榴皮苷的活性成分。采用流式细胞术和蛋白质免疫印迹法评估OGD/R和/或石榴皮苷对细胞周期阻滞的影响。应用免疫荧光分析法评估细胞核转位。使用酶联免疫吸附测定法检测活性氧（ROS）和谷胱甘肽（GSH）的相对含量以及过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的酶活性。

结果

生物活性导向分离数据表明，[植物名称]中的石榴皮苷可减轻OGD/R诱导的SH-SY5Y细胞损伤。流式细胞术分析和蛋白质免疫印迹法检测细胞周期蛋白依赖性激酶1（CDK1）、磷酸化CDK1（p-CDK1）、细胞周期蛋白B1（cyclin B1）和p21的表达，结果显示石榴皮苷可缓解OGD/R诱导的细胞周期G0/G1期阻滞。此外，免疫荧光分析法和蛋白质免疫印迹法检测转化生长因子β（TGF-β）和磷酸化Smad2/磷酸化Smad3（p-Smad2/p-Smad3）的表达，结果显示石榴皮苷可缓解OGD/R诱导的TGF-β/Smad信号通路。此外，使用LY2157299（一种TGF-β/Smad信号通路抑制剂）证实TGF-β/Smad信号通路对石榴皮苷的作用至关重要。最后，结果表明石榴皮苷可缓解OGD/R诱导的氧化应激。

结论

从[植物名称]中鉴定出的活性成分石榴皮苷是一种减轻OGD/R诱导的细胞损伤的保护剂，其可通过TGF-β/Smad信号通路调节氧化应激和细胞周期阻滞在SH-SY5Y细胞中发挥保护作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f649/7895578/bc45e627a211/ECAM2021-6671282.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f649/7895578/f7932f34d975/ECAM2021-6671282.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f649/7895578/3046afb25133/ECAM2021-6671282.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f649/7895578/ded24372a060/ECAM2021-6671282.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f649/7895578/f03f6ec063a0/ECAM2021-6671282.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f649/7895578/e167690eb370/ECAM2021-6671282.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f649/7895578/0b6b96d7faf8/ECAM2021-6671282.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f649/7895578/bc45e627a211/ECAM2021-6671282.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f649/7895578/f7932f34d975/ECAM2021-6671282.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f649/7895578/3046afb25133/ECAM2021-6671282.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f649/7895578/ded24372a060/ECAM2021-6671282.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f649/7895578/f03f6ec063a0/ECAM2021-6671282.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f649/7895578/e167690eb370/ECAM2021-6671282.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f649/7895578/0b6b96d7faf8/ECAM2021-6671282.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f649/7895578/bc45e627a211/ECAM2021-6671282.007.jpg

相似文献

1
Punicalin Alleviates OGD/R-Triggered Cell Injury via TGF--Mediated Oxidative Stress and Cell Cycle in Neuroblastoma Cells SH-SY5Y.石榴皮苷通过TGF-β介导的氧化应激和细胞周期减轻氧糖剥夺/复氧触发的神经母细胞瘤细胞SH-SY5Y损伤。
Evid Based Complement Alternat Med. 2021 Feb 12;2021:6671282. doi: 10.1155/2021/6671282. eCollection 2021.
2
Artemisinin Alleviates Cerebral Ischemia/Reperfusion-Induced Oxidative Damage via Regulating PHB2-Mediated Autophagy in the Human Neuroblastoma SH-SY5Y Cell Line.青蒿素通过调节 PHB2 介导线粒体自噬减轻人神经母细胞瘤 SH-SY5Y 细胞脑缺血/再灌注诱导的氧化损伤。
Oxid Med Cell Longev. 2022 Dec 15;2022:6568748. doi: 10.1155/2022/6568748. eCollection 2022.
3
Erratum to "Punicalin Alleviates OGD/R-Triggered Cell Injury via TGF--Mediated Oxidative Stress and Cell Cycle in Neuroblastoma Cells SH-SY5Y.《石榴皮苷通过TGF-β介导的氧化应激和细胞周期减轻氧糖剥夺/复氧触发的神经母细胞瘤细胞SH-SY5Y损伤》勘误
Evid Based Complement Alternat Med. 2021 Apr 19;2021:9840240. doi: 10.1155/2021/9840240. eCollection 2021.
4
Physcion Protects Rats Against Cerebral Ischemia-Reperfusion Injury via Inhibition of TLR4/NF-kB Signaling Pathway.大黄素通过抑制 TLR4/NF-κB 信号通路保护大鼠免受脑缺血再灌注损伤。
Drug Des Devel Ther. 2021 Jan 25;15:277-287. doi: 10.2147/DDDT.S267856. eCollection 2021.
5
Inhibition of ERK downregulates autophagy via mitigating mitochondrial fragmentation to protect SH-SY5Y cells from OGD/R injury.抑制 ERK 通过减轻线粒体碎片化来下调自噬，从而保护 SH-SY5Y 细胞免受 OGD/R 损伤。
Cell Commun Signal. 2023 Aug 14;21(1):204. doi: 10.1186/s12964-023-01211-3.
6
Protective effect of Cornuside on OGD/R injury in SH-SY5Y cells and its underlying mechanism.山茱萸新苷对 OGD/R 损伤 SH-SY5Y 细胞的保护作用及其机制研究。
Brain Res. 2023 Dec 15;1821:148585. doi: 10.1016/j.brainres.2023.148585. Epub 2023 Sep 16.
7
Upregulation of miR-22 alleviates oxygen-glucose deprivation/reperfusion-induced injury by targeting Tiam1 in SH-SY5Y cells.miR-22 的上调通过靶向 SH-SY5Y 细胞中的 Tiam1 缓解氧葡萄糖剥夺/再灌注诱导的损伤。
Acta Biochim Pol. 2023 Sep 6;70(3):495-501. doi: 10.18388/abp.2020_6189.
8
LncRNA SNHG12 as a potent autophagy inducer exerts neuroprotective effects against cerebral ischemia/reperfusion injury.长链非编码 RNA SNHG12 作为一种有效的自噬诱导剂，对脑缺血/再灌注损伤发挥神经保护作用。
Biochem Biophys Res Commun. 2019 Jun 25;514(2):490-496. doi: 10.1016/j.bbrc.2019.04.158. Epub 2019 May 2.
9
Neuroprotective effect of FMS-like tyrosine kinase-3 silence on cerebral ischemia/reperfusion injury in a SH-SY5Y cell line.FMS 样酪氨酸激酶 3 沉默对 SH-SY5Y 细胞脑缺血/再灌注损伤的神经保护作用。
Gene. 2019 May 20;697:152-158. doi: 10.1016/j.gene.2019.01.043. Epub 2019 Feb 15.
10
Endoplasmic reticulum stress regulates oxygen-glucose deprivation-induced parthanatos in human SH-SY5Y cells via improvement of intracellular ROS.内质网应激通过改善细胞内 ROS 调节氧葡萄糖剥夺诱导的人 SH-SY5Y 细胞 parthanatos
CNS Neurosci Ther. 2018 Jan;24(1):29-38. doi: 10.1111/cns.12771. Epub 2017 Oct 16.

引用本文的文献

1
Curcumin in vitro Neuroprotective Effects Are Mediated by p62/keap-1/Nrf2 and PI3K/AKT Signaling Pathway and Autophagy Inhibition.姜黄素的体外神经保护作用由p62/keap-1/Nrf2和PI3K/AKT信号通路以及自噬抑制介导。
Physiol Res. 2023 Aug 31;72(4):497-510. doi: 10.33549/physiolres.935054.
2
Neuronal Responses to Ischemia: Scoping Review of Insights from Human-Derived In Vitro Models.神经元对缺血的反应：来自人源体外模型的见解的范围综述。
Cell Mol Neurobiol. 2023 Oct;43(7):3137-3160. doi: 10.1007/s10571-023-01368-y. Epub 2023 Jun 28.
3
The Antimicrobial Effect of Pomegranate Peel Extract versus Chlorhexidine in High Caries Risk Individuals Using Quantitative Real-Time Polymerase Chain Reaction: A Randomized Triple-Blind Controlled Clinical Trial.

本文引用的文献

1
Procyanidins exhibits neuroprotective activities against cerebral ischemia reperfusion injury by inhibiting TLR4-NLRP3 inflammasome signal pathway.原花青素通过抑制 TLR4-NLRP3 炎性小体信号通路发挥对脑缺血再灌注损伤的神经保护作用。
Psychopharmacology (Berl). 2020 Nov;237(11):3283-3293. doi: 10.1007/s00213-020-05610-z. Epub 2020 Jul 29.
2
Propolis ameliorates cerebral injury in focal cerebral ischemia/reperfusion (I/R) rat model via upregulation of TGF-β1.蜂胶通过上调转化生长因子-β1改善局灶性脑缺血/再灌注（I/R）大鼠模型中的脑损伤。
Saudi Pharm J. 2020 Jan;28(1):116-126. doi: 10.1016/j.jsps.2019.11.013. Epub 2019 Dec 7.
3
使用定量实时聚合酶链反应比较石榴皮提取物与氯己定对高龋风险个体的抗菌作用：一项随机三盲对照临床试验
Int J Dent. 2021 Aug 30;2021:5563945. doi: 10.1155/2021/5563945. eCollection 2021.
4
Erratum to "Punicalin Alleviates OGD/R-Triggered Cell Injury via TGF--Mediated Oxidative Stress and Cell Cycle in Neuroblastoma Cells SH-SY5Y.《石榴皮苷通过TGF-β介导的氧化应激和细胞周期减轻氧糖剥夺/复氧触发的神经母细胞瘤细胞SH-SY5Y损伤》勘误
Evid Based Complement Alternat Med. 2021 Apr 19;2021:9840240. doi: 10.1155/2021/9840240. eCollection 2021.
Qingkailing injection ameliorates cerebral ischemia-reperfusion injury and modulates the AMPK/NLRP3 Inflammasome Signalling pathway.
清开灵注射液改善脑缺血再灌注损伤并调节 AMPK/NLRP3 炎性小体信号通路。
BMC Complement Altern Med. 2019 Nov 20;19(1):320. doi: 10.1186/s12906-019-2703-5.
4
TGF-β2/Smad3 Signaling Pathway Activation Through Enhancing VEGF and CD34 Ameliorates Cerebral Ischemia/Reperfusion Injury After Isoflurane Post-conditioning in Rats.TGF-β2/Smad3 信号通路的激活通过增强 VEGF 和 CD34 减轻异氟醚后处理大鼠脑缺血/再灌注损伤。
Neurochem Res. 2019 Nov;44(11):2606-2618. doi: 10.1007/s11064-019-02880-8. Epub 2019 Sep 25.
5
Pharmacodynamic Effect of Ellagic Acid on Ameliorating Cerebral Ischemia/Reperfusion Injury.鞣花酸改善脑缺血/再灌注损伤的药效学作用。
Pharmacology. 2019;104(5-6):320-331. doi: 10.1159/000502401. Epub 2019 Aug 30.
6
α-Tocopherol preserves cardiac function by reducing oxidative stress and inflammation in ischemia/reperfusion injury.α-生育酚通过减少缺血/再灌注损伤中的氧化应激和炎症来保护心脏功能。
Redox Biol. 2019 Sep;26:101292. doi: 10.1016/j.redox.2019.101292. Epub 2019 Aug 6.
7
Putting the brakes on the cell cycle: mechanisms of cellular growth arrest.抑制细胞周期：细胞生长抑制的机制。
Curr Opin Cell Biol. 2019 Oct;60:106-113. doi: 10.1016/j.ceb.2019.05.005. Epub 2019 Jun 25.
8
YiQi Tongluo Granule against Cerebral Ischemia/Reperfusion Injury in Rats by Freezing GluN2B and CaMK II through NMDAR/ERK1/2 Signaling.益气通络颗粒通过NMDAR/ERK1/2信号通路冻结GluN2B和CaMK II抗大鼠脑缺血/再灌注损伤
Chem Pharm Bull (Tokyo). 2019 Mar 1;67(3):244-252. doi: 10.1248/cpb.c18-00806. Epub 2018 Dec 28.
9
Ischemia-Reperfusion Injury After Endovascular Thrombectomy for Ischemic Stroke.血管内血栓切除术治疗缺血性脑卒中后的缺血再灌注损伤。
Stroke. 2018 Dec;49(12):3071-3074. doi: 10.1161/STROKEAHA.118.022015.
10
Dioscin ameliorates intestinal ischemia/reperfusion injury via adjusting miR-351-5p/MAPK13-mediated inflammation and apoptosis.薯蓣皂苷通过调节 miR-351-5p/MAPK13 介导的炎症和凋亡改善肠缺血再灌注损伤。
Pharmacol Res. 2019 Jan;139:431-439. doi: 10.1016/j.phrs.2018.11.040. Epub 2018 Nov 29.