Park Won Seo, Park Min Su, Kang Sang Wook, Jin Seul A, Jeon Youngchul, Hwang Jeikiun, Kim Su Kang
Department of Surgery, School of Medicine, Kyung Hee University, Seoul, Republic of Korea.
Department of Dental Pharmacology, College of Dentistry, Dankook University, Cheonan, Republic of Korea.
Transplant Proc. 2019 Oct;51(8):2838-2841. doi: 10.1016/j.transproceed.2019.02.055. Epub 2019 Sep 4.
Hesperidin is a well-known flavanone glycoside copiously found in sweet orange and lemon, which was recently reported to possess significant anti-inflammatory, analgesic, antifungal, antiviral, antioxidant, and anticancer activities. Ischemia-reperfusion (I/R) injury is a major problem after renal transplantation. Furthermore, inflammatory responses to I/R exacerbate the resultant renal injury. In the present study, we investigated whether hesperidin exhibits renoprotective effects against I/R-induced acute kidney injury in a rat model.
We fed Sprague-Dawley rats either hesperidin (100 mg/kg/d) or saline. One week later, ischemia was induced by bilateral renal pedicle occlusion for 30 minutes followed by reperfusion. The rats were randomly divided into 3 groups, which were treated as follows: 1. the sham operated group; 2. the I/R group; 3. the I/R-hesperidin group RESULTS: Compared to the sham group, the I/R group had higher expression of blood urea nitrogen and serum creatinine and lower expression of catalase, superoxide dismutase, glutathione peroxidase, antioxidants, and nitric oxide. Compared to the I/R group, the I/R-hesperidin group had higher expression of catalase, superoxide dismutase, glutathione peroxidase, antioxidant, and nitric oxide and lower expression of blood urea nitrogen and serum creatinine.
Hesperidin improved acute renal I/R injury through its antioxidant effects. These findings suggest that hesperidin is a potential therapeutic agent for acute ischemia-induced renal damage.
橙皮苷是一种在甜橙和柠檬中大量存在的著名黄酮糖苷，最近有报道称其具有显著的抗炎、镇痛、抗真菌、抗病毒、抗氧化和抗癌活性。缺血再灌注（I/R）损伤是肾移植后的一个主要问题。此外，对I/R的炎症反应会加剧由此导致的肾损伤。在本研究中，我们调查了橙皮苷在大鼠模型中是否对I/R诱导的急性肾损伤具有肾保护作用。
我们给斯普拉格-道利大鼠喂食橙皮苷（100mg/kg/d）或生理盐水。一周后，通过双侧肾蒂闭塞30分钟诱导缺血，随后进行再灌注。大鼠被随机分为3组，治疗如下：1.假手术组；2.I/R组；3.I/R-橙皮苷组。结果：与假手术组相比，I/R组血尿素氮和血清肌酐表达较高，而过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、抗氧化剂和一氧化氮表达较低。与I/R组相比，I/R-橙皮苷组过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、抗氧化剂和一氧化氮表达较高，血尿素氮和血清肌酐表达较低。
橙皮苷通过其抗氧化作用改善急性肾I/R损伤。这些发现表明橙皮苷是急性缺血性肾损伤的一种潜在治疗药物。
