Abbasi Hadis, Ranjbar Reza
MSC Microbiology Department, Islamic Azad University, North Tehran Branch, Tehran, Iran.
Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Cent European J Urol. 2018;71(1):129-133. doi: 10.5173/ceju.2018.1539. Epub 2017 Jan 18.
(E.coli) as an opportunistic pathogen is a major cause of the hospital infections. The main goal of this research was to determine the frequency of quinolone resistance genes (qnr) among E.coli pathotypes isolated from patients with urinary tract infections (UTIs).
Urine samples were obtained from patients with UTIs in three major hospitals of Mofid, Bu Ali, and Vali-Asr during the year of 2015 in Tehran, Iran. The antibiogram was done for isolated bacterial isolates using nalidixic acid, norfloxacin, gentamicin, streptomycin, and chloramphenicol. Then the plasmids of the bacterial samples were extracted. PCR was used to detect qnr genes. Finally, the PCR products were run on a 1% agarose gel electrophoresis and the results were analyzed by the program SPSS version 22.
Overall, 100 E.coli strains were isolated from patients with UTIs. The highest resistance rate was against Streptomycin. The frequency of the genes of qnrA, qnrB and qnrS were 0%, 25% and 36%, respectively. Moreover, the presence of the both genes of qnrB and qnrS was recognized in 10% of isolated bacterial strains.
Our results indicated increasing rates of quinolone resistant E.coli strains circulating in hospitals under the study. Dissemination of these strains harboring qnr determinants is of particular concern.
大肠埃希菌作为一种机会致病菌，是医院感染的主要原因。本研究的主要目的是确定从尿路感染（UTIs）患者分离出的大肠埃希菌致病型中喹诺酮耐药基因（qnr）的频率。
2015年在伊朗德黑兰的莫菲德、布阿里和瓦利 - 阿斯尔三大医院，从尿路感染患者中获取尿液样本。对分离出的细菌菌株使用萘啶酸、诺氟沙星、庆大霉素、链霉素和氯霉素进行药敏试验。然后提取细菌样本的质粒。采用聚合酶链反应（PCR）检测qnr基因。最后，将PCR产物在1%琼脂糖凝胶上进行电泳，并使用SPSS 22版程序分析结果。
总体而言，从尿路感染患者中分离出100株大肠埃希菌菌株。耐药率最高的是链霉素。qnrA、qnrB和qnrS基因的频率分别为0%、25%和36%。此外，在10%的分离细菌菌株中发现了qnrB和qnrS两个基因。
我们的结果表明，在本研究的医院中，喹诺酮耐药大肠埃希菌菌株的比例在增加。携带qnr决定簇的这些菌株的传播尤其令人担忧。
