Detection of Plasmid-Mediated Genes Among the Clinical Quinolone-Resistant Strains Isolated in Tehran, Iran.

BACKGROUND

is one of the most important bacterial agents to cause urinary tract infections. Inappropriate and unnecessary administration of antibiotics has led to an increase in the appearance of multidrug-resistant isolates, limiting treatment options. The increase in a number of resistant strains of bacteria is a major concern of health authorities worldwide.

OBJECTIVE

The purpose of this study was to determine the presence of the genes among isolated from UTIs of patients in Baqiyatallah hospital in Tehran province, Iran.

METHOD

Clinical urine samples of patients with suspected urinary tract infection were collected by standard methods in sterile disposable containers. After analysis of urine, microscopic observations and culture analysis, the bacterial genome was extracted by boiling method. PCR for detection of genes including , and was done by specific primers, then PCR products were run using gel electrophoresis and visualized by gel documentation system.

RESULTS

In the present study among the 95 isolates, 60 strains were resistant to nalidixic acid. PCR showed that 92 strains were positive for . The and genes were not found among the clinical isolates.

CONCLUSION

Our finding indicates a high level of resistance against nalidixic acid among isolates recovered from the patients with UTI. Also, the high frequency of imposes the importance of survey of molecular and genetic analysis of mechanisms of quinolone resistance in strains.