Li Fay-Wei, Harkess Alex
Boyce Thompson Institute Ithaca New York 14853 USA.
Plant Biology Section Cornell University Ithaca New York 14853 USA.
Appl Plant Sci. 2018 Mar 30;6(3):e1030. doi: 10.1002/aps3.1030. eCollection 2018 Mar.
With the rapid development of sequencing technology and the plummeting cost, assembling whole genomes from non-model plants will soon become routine for plant systematists and evolutionary biologists. Here we summarize and compare several of the latest genome sequencing and assembly approaches, offering a practical guide on how to approach a genome project. We also highlight certain precautions that need to be taken before investing time and money into a genome project.
随着测序技术的迅速发展和成本的大幅下降,从非模式植物中组装全基因组对于植物分类学家和进化生物学家来说很快将成为常规操作。在此,我们总结并比较了几种最新的基因组测序和组装方法,提供了关于如何开展基因组项目的实用指南。我们还强调了在投入时间和资金开展基因组项目之前需要采取的某些预防措施。