Yang Lin, Chen Ya Mei, He Run Lian, Deng Chang Chun, Liu Jun Wei, Liu Yang
Long-term Research Station of Alpine Forest Ecosystems/Key Laboratory of Forestry Ecological Engineering in the Upper Reaches of Yangtze River/Institute of Ecology & Forestry, Sichuan Agricultural University, Chengdu 611130, China.
Ying Yong Sheng Tai Xue Bao. 2016 Sep;27(9):2855-2863. doi: 10.13287/j.1001-9332.201609.026.
Soil samples were collected from an alpine coniferous forest. Soil cores with soil organic layer above and mineral soil layer below were incubated in plant growth chambers during 10 weeks. Taking the annual average soil temperature in the alpine forest as the control, and other two levels of temperature were increased 2 and 4 ℃, respectively, to investigate the responses of soil microbial community and soil enzyme activity to warming. The results showed that warming significantly reduced PLFAs content of bacteria in soil organic layer and PLFAs content of G in the mineral soil layer, whereas soil fungi was slightly influenced by warming. Warming caused the G/G increasing and changed the microbial community structure, but had no significant effect on soil enzymes activity, i.e., laccase (Lac), β-1,4-glucoside (BG), acid phosphate (AP), β-1,4-N-acetylglucosaminidase (NAG). There were significantly positive correlations between fungi, bacteria, G and G, showing the coordinated growth trend between soil microbial communities. There was significantly negative correlation between BG and bacteria, showing the BG competing for carbon source utilization with soil microbial community. In consequence, the soil microbe types responded differently to warming. Bacteria were more sensitive to temperature than fungi, and fungi had stronger ability to tolerate warming in the alpine forest.
从高山针叶林采集土壤样本。将带有上方土壤有机层和下方矿质土壤层的土芯在植物生长室中培养10周。以高山森林的年平均土壤温度为对照，另外两个温度水平分别升高2℃和4℃，以研究土壤微生物群落和土壤酶活性对变暖的响应。结果表明，变暖显著降低了土壤有机层中细菌的磷脂脂肪酸（PLFAs）含量以及矿质土壤层中革兰氏阴性菌（G）的PLFAs含量，而土壤真菌受变暖的影响较小。变暖导致G/G增加并改变了微生物群落结构，但对土壤酶活性没有显著影响，即漆酶（Lac）、β-1,4-葡萄糖苷酶（BG）、酸性磷酸酶（AP）、β-1,4-N-乙酰氨基葡萄糖苷酶（NAG）。真菌、细菌、G和G之间存在显著正相关，表明土壤微生物群落之间存在协同生长趋势。BG与细菌之间存在显著负相关，表明BG与土壤微生物群落竞争碳源利用。因此，土壤微生物类型对变暖的响应不同。在高山森林中，细菌比真菌对温度更敏感，而真菌具有更强的耐变暖能力。
