Memon K, Cheng Y, Panhwar F, Chen Z R, Haider Z, Afridi S, Hu P, Zhao G
Department of Electronic Science and Technology, University of Science and Technology of China.
Department of Precision Instruments, Tsinghua University Graduate School at Shenzhen, Shenzhen, China.
Cryo Letters. 2018 Jan/Feb;39(1):7-13.
BACKGROUND: Osmotic shock upon the addition and removal of cryoprotectant agent (CPA) is a major source of cell damage during cryopreservation.
Microfluidic device offers a new platform for CPA loading and unloading. The micro scale dimension makes possible to perform a detailed analysis and controllable removal of CPA with many advantages.
A microfluidic device was developed for extracting dimethyl sulfoxide (DMSO) from the sample streamline. The device has two parallel channels separated by a polytetrafluoroethylene (PTFE) membrane and serves as the stable environment for CPA removal. A diffusion-based simulation model was used to characterize the CPA extraction. To support the experimental design and device optimization we developed analogous scheme to simulate by COMSOL Multiphysics.
The device can extract cryoprotectant in a mesoscale volume from cells and simplify the post-thaw sample handling. It has sufficient control on loading/unloading of CPAs by controlling the flow rate of cell stream/wash stream solutions via syringe pumps. Compared to other customary devices, this device is easy to fabricate and assemble, with features of high precision, reusability and low cost.
背景：在添加和去除冷冻保护剂（CPA）时的渗透压冲击是冷冻保存过程中细胞损伤的主要来源。
微流控装置为CPA的加载和卸载提供了一个新平台。微尺度尺寸使得对CPA进行详细分析和可控去除成为可能，且具有许多优势。
开发了一种用于从样品流中提取二甲基亚砜（DMSO）的微流控装置。该装置有两个由聚四氟乙烯（PTFE）膜隔开的平行通道，为CPA去除提供稳定环境。使用基于扩散的模拟模型来表征CPA提取过程。为支持实验设计和装置优化，我们开发了类似方案，通过COMSOL Multiphysics进行模拟。
该装置能够从细胞中提取中尺度体积的冷冻保护剂，并简化解冻后样品处理。通过注射泵控制细胞流/洗涤流溶液流速，对CPA的加载/卸载有足够的控制能力。与其他传统装置相比，该装置易于制造和组装，具有高精度、可重复使用和低成本的特点。
