补充抗冻蛋白对低温保存猪桑椹胚发育的影响。
Effects of Antifreeze Protein Supplementation on the Development of Porcine Morulae Stored at Hypothermic Temperatures.
作者信息
Nguyen T V, Tanihara F, Hirata M, Hirano T, Nishio K, Kim Do L T, Nguyen T V, Nii M, Otoi T
机构信息
Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan.
Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan. Faculty of Veterinary Science, Vietnam National University of Agriculture, Vietnam.
出版信息
Cryo Letters. 2018 Mar/Apr;39(2):131-136.
BACKGROUND
Short-term storage is valuable method to reuse manipulated embryos.
OBJECTIVE
The present study evaluated the effects of antifreeze protein (AFP) supplementation on the quality and development of in vitro-produced porcine morulae after short-term storage (24 h).
MATERIALS AND METHODS
The morulae were stored with various concentrations of AFP type III for 24 h at 5, 15 and 25C.
RESULTS
Supplementation of AFP type III (1.0 microgram per mL) improved the developmental competence of embryos stored at 25C. The proportions of DNA-fragmented nuclei in the blastocysts did not differ between the embryos stored at 25C and the control embryos without storage treatment. However, the developmental competence of embryos stored at hypothermic temperatures decreased relative to that of the control embryos.
CONCLUSION
Supplementation of AFP type III (1.0 microgram per mL) maintained the quality of embryos stored at 25C, but did not have beneficial effects on the development of embryos stored at hypothermic temperatures.
背景
短期储存是重复利用操作过的胚胎的一种有价值的方法。
目的
本研究评估了添加抗冻蛋白（AFP）对体外生产的猪桑椹胚短期储存（24小时）后的质量和发育的影响。
材料与方法
将桑椹胚与不同浓度的III型AFP在5℃、15℃和25℃下储存24小时。
结果
添加III型AFP（每毫升1.0微克）提高了在25℃下储存的胚胎的发育能力。在25℃下储存的胚胎与未进行储存处理的对照胚胎相比，囊胚中DNA片段化细胞核的比例没有差异。然而，与对照胚胎相比，低温储存的胚胎的发育能力下降。
结论
添加III型AFP（每毫升1.0微克）可维持在25℃下储存的胚胎的质量，但对低温储存的胚胎的发育没有有益影响。
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