Key Laboratory of Crop Heterosis and Utilization (MOE)/Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, China.
Pest Manag Sci. 2018 Dec;74(12):2754-2760. doi: 10.1002/ps.5062. Epub 2018 Jun 29.
Chitin is an important component of the insect exoskeleton and peritrophic membrane. Chitin synthase 1 (CHS1) is a key enzyme in the chitin synthesis pathway, and has a role in insect molting and growth. Plant-mediated RNA interference (RNAi) has been used as a more target-specific and environmentally safe approach to prevent and control agricultural insects. The aims of this study were to use grain aphid (Sitobion avanae) CHS1 as the target gene and to produce transgenic wheat lines for aphid control via plant-mediated RNAi.
Expression levels of CHS1 changed at different developmental stages. After feeding on the representative T transgenic lines Tb5-2 and Tb10-3, CHS1 expression levels in grain aphid decreased by 50.29% and 45.32%, respectively; and total and molting aphid numbers reduced significantly, compared with controls. Consistent with this, aphid numbers in mixed natural populations reduced significantly in the respective T and T transgenic lines under field conditions, and T5 transgenic lines had higher grain weight compared with the unsprayed insecticide wild-type and insecticide-sprayed wild-type.
These results indicate that plant-mediated RNAi of the grain aphid CHS1 gene confers common wheat resistance against aphids. © 2018 Society of Chemical Industry.
几丁质是昆虫外骨骼和围食膜的重要组成部分。几丁质合酶 1（CHS1）是几丁质合成途径中的关键酶，在昆虫蜕皮和生长中起作用。植物介导的 RNA 干扰（RNAi）已被用作一种更具靶向性和更安全的方法来预防和控制农业害虫。本研究旨在以麦长管蚜 CHS1 为靶基因，通过植物介导的 RNAi 生产抗蚜转基因小麦品系。
CHS1 的表达水平在不同的发育阶段发生变化。在取食代表性的 T 转基因株系 Tb5-2 和 Tb10-3 后，麦长管蚜 CHS1 的表达水平分别下降了 50.29%和 45.32%；与对照相比，总蚜虫和蜕皮蚜虫的数量显著减少。与此一致的是，在田间条件下，各自的 T 和 T 转基因系中混合自然种群中的蚜虫数量显著减少，T5 转基因系的籽粒重量高于未喷洒杀虫剂的野生型和喷洒杀虫剂的野生型。
这些结果表明，植物介导的麦长管蚜 CHS1 基因的 RNAi 赋予普通小麦抗蚜性。© 2018 化学工业协会。
