Ahmad Shakil, Jamil Momana, Fahim Muhammad, Zhang Shujing, Ullah Farman, Lyu Baoqian, Luo Yanping
School of Plant Protection, Hainan University, Haikou, China.
Centre for Omic Sciences, Islamia College University, Peshawar, Pakistan.
Front Genet. 2021 Jul 5;12:691382. doi: 10.3389/fgene.2021.691382. eCollection 2021.
This study reports the first successful use of oral feeding dsRNA technique for functional characterization of imaginal disc growth factors (IDGFs) genes (, , , , and ) in melon fly Phylogenetic and domain analysis indicates that these genes had high similarity with other Tephritidae fruit flies homolog and contain only one conserved domain among these five genes, which is glyco-18 domain (glyco-hydro-18 domain). Gene expression analysis at different developmental stages revealed that these genes were expressed at larval, pupal, and adult stages. To understand their role in different developmental stages, larvae were fed dsRNA-corresponding to each of the five IDGFs, in an artificial diet. RNAi-mediated knockdown of shows no phenotypic effects but caused mortality (10.4%), while caused malformed pharate at the adult stage where insects failed to shed their old cuticle and remained attached with their body, highest mortality (49.2%) was recorded compared to dsRNA-green fluorescent protein (GFP) or DEPC. Silencing of and cause lethal phenotype in larvae, (17.2%) and (40%) mortality was indexed in . was mainly expressed in pupae and adult stages, and its silencing caused a malformation in adult wings. The developmental defects such as malformation in wings, larval-larval lethality, pupal-adult malformation, and small body size show that IDGFs are key developmental genes in the melon fly. Our results provide a baseline for the melon fly management and understanding of IDGFs specific functions in
本研究报告了首次成功使用口服喂食双链RNA技术对瓜实蝇中imaginal disc生长因子（IDGFs）基因（、、、和）进行功能表征。系统发育和结构域分析表明，这些基因与其他实蝇科果蝇的同源基因具有高度相似性，并且这五个基因中仅包含一个保守结构域，即糖基-18结构域（糖基水解-18结构域）。不同发育阶段的基因表达分析表明，这些基因在幼虫、蛹和成虫阶段均有表达。为了解它们在不同发育阶段的作用，在人工饲料中给幼虫喂食对应于五个IDGFs中每一个的双链RNA。RNA干扰介导的基因敲低未显示出表型效应，但导致了死亡率（10.4%），而基因敲低导致成虫阶段出现畸形，昆虫无法蜕去旧表皮并与身体相连，与双链RNA-绿色荧光蛋白（GFP）或DEPC相比，记录到最高死亡率（49.2%）。基因和的沉默在幼虫中导致致死表型，基因沉默的死亡率为（17.2%），基因沉默的死亡率为（40%）。基因主要在蛹期和成虫期表达，其沉默导致成虫翅膀畸形。翅膀畸形、幼虫-幼虫致死、蛹-成虫畸形和体型小等发育缺陷表明，IDGFs是瓜实蝇中的关键发育基因。我们的结果为瓜实蝇的管理以及了解IDGFs在中的特定功能提供了基线
