University of Bern, Institute of Applied Physics, Department of Biomedical Photonics, Sidlerstrasse 5, Bern, 3012, Switzerland. University Medicine Rostock, Department of Cell Biology, Schillingallee 69, Rostock, D-18057, Germany.
Biomed Mater. 2018 May 29;13(5):055001. doi: 10.1088/1748-605X/aac332.
Electrospun meshes have been extensively investigated for tissue engineering and drug delivery. The application of this technology is of interest for laser-assisted vascular anastomosis (LAVA) due to the possibility to bind and stabilize macromolecules in fibers.
We prepared bovine serum albumin (BSA) blend microfibers from the auxiliary proteins polyethylene oxide (PEO), polycaprolactone (PCL), polyvinyl alcohol (PVA) and gelatin. The thickness and weight of the resulting patches were measured and the morphological characteristics were observed by scanning electron microscopy. Thereafter, layered patches were prepared by spinning the BSA/polymer layer on top of a light absorbing layer made of indocyanine green and PCL. The effect of the material composition of the electrospun patches on the behavior during LAVA, the bonding strength and the resulting thermal damage were investigated.
The bonding strength of the tissue fusion increased with higher BSA amounts in the patch. By using PEO, a ratio of 85/15 (w/w) of BSA/PEO was stable during electrospinning, leading to a shear strength that was similar to patches that were soaked in liquid BSA (20.7 ± 4.1 mN mm and 20.3 ± 4.1 mN mm, respectively). The handling during LAVA was however drastically improved by using a layered patch made from BSA/PEO. Thermal damage was similar compared to previous solder materials.
This study investigated the maximum amount of BSA possible in electrospun polymer fibers made from PEO, PCL, PVA and gelatin. Both, the process of electrospinning and the performance during ex vivo LAVA, makes the BSA/PEO blend a promising material for LAVA.
静电纺丝网已广泛应用于组织工程和药物输送。由于该技术有可能在纤维中结合和稳定大分子，因此它在激光辅助血管吻合术（LAVA）中的应用引起了人们的兴趣。
我们从辅助蛋白聚乙烯氧化物（PEO）、聚己内酯（PCL）、聚乙烯醇（PVA）和明胶中制备了牛血清白蛋白（BSA）共混微纤维。测量了所得贴片的厚度和重量，并通过扫描电子显微镜观察了其形态特征。然后，通过在由吲哚菁绿和 PCL 制成的光吸收层上纺制 BSA/聚合物层，制备了分层贴片。研究了静电纺丝贴片的材料组成对 LAVA 过程中的行为、结合强度和产生的热损伤的影响。
贴片的 BSA 含量越高，组织融合的结合强度越高。使用 PEO 时，BSA/PEO 的比例为 85/15（w/w），在静电纺丝过程中稳定，导致其剪切强度与浸泡在液态 BSA 中的贴片相似（分别为 20.7±4.1 mN mm 和 20.3±4.1 mN mm）。然而，使用由 BSA/PEO 制成的分层贴片大大改善了 LAVA 过程中的处理性能。与以前的焊接材料相比，热损伤相似。
本研究调查了 PEO、PCL、PVA 和明胶制成的静电纺丝聚合物纤维中可能存在的最大 BSA 量。静电纺丝过程和体外 LAVA 过程中的性能都使 BSA/PEO 混合物成为 LAVA 的一种有前途的材料。
