Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Gambang 26300, Pahang, Malaysia.
Centre of Excellence for Advanced Research in Fluid Flow (CARIFF), Universiti Malaysia Pahang, Gambang 26300, Pahang, Malaysia.
Curr Drug Deliv. 2019;16(10):913-922. doi: 10.2174/1567201816666191029122445.
This paper presents the effect of solution properties and operating parameters of polyethylene oxide (PEO) based nanofiber using a wire electrode-based needleless electrospinning.
The feed solution was prepared using a PEO dissolved in water or a water-ethanol mixture. The PEO solution is blended with Bovine Serum Albumin protein (BSA) as a model drug to study the effect of the electrospinning process on the stability of the loaded protein. The polymer solution properties such as viscosity, surface tension, and conductivity were controlled by adjusting the solvent and salt content. The morphology and fiber size distribution of the nanofiber was analyzed using scanning electron microscopy.
The results show that the issue of a beaded nanofiber can be eliminated either by increasing the solution viscosity or by the addition of salt and ethanol to the PEO-water system. The addition of salt and solvent produced a high frequency of smaller fiber diameter ranging from 100 to 150 nm. The encapsulation of BSA in PEO nanofiber was characterized by three different spectroscopy techniques (i.e. circular dichroism, Fourier transform infrared, and fluorescence) and the results showed the BSA is well encapsulated in the PEO matrix with no changes in the protein structure.
This work may serve as a useful guide for a drug delivery industry to process a nanofiber at a large and continuous scale with a blend of drugs in nanofiber using a wire electrode electrospinning.
本文介绍了基于聚乙烯氧化物（PEO）的纳米纤维在基于线状电极的无针电纺过程中的溶液性质和操作参数的影响。
采用 PEO 溶解在水中或水-乙醇混合物中的方法制备了进料溶液。PEO 溶液与牛血清白蛋白（BSA）混合作为模型药物，以研究电纺过程对负载蛋白质稳定性的影响。通过调整溶剂和盐含量来控制聚合物溶液的性质，如粘度、表面张力和电导率。使用扫描电子显微镜分析了纳米纤维的形态和纤维尺寸分布。
结果表明，通过增加溶液的粘度或向 PEO-水系统中添加盐和乙醇，可以消除珠状纳米纤维的问题。添加盐和溶剂会产生更高频率的较小纤维直径，范围从 100 到 150nm。通过三种不同的光谱技术（即圆二色性、傅里叶变换红外和荧光）对 PEO 纳米纤维中 BSA 的包封进行了表征，结果表明 BSA 很好地包封在 PEO 基质中，蛋白质结构没有变化。
这项工作可以为药物输送行业提供有用的指导，以使用线状电极电纺在纳米纤维中混合药物，在大规模和连续的基础上处理纳米纤维。
