利用工程化甲基杆菌（Methylobacterium extorquens）将 CO 电化学转化为增值产品甲酸盐。
Bioelectrochemical conversion of CO to value added product formate using engineered Methylobacterium extorquens.
机构信息
School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), 50 UNIST-gil, Ulsan, 44919, Republic of Korea.
出版信息
Sci Rep. 2018 May 8;8(1):7211. doi: 10.1038/s41598-018-23924-z.
The conversion of carbon dioxide to formate is a fundamental step for building C1 chemical platforms. Methylobacterium extorquens AM1 was reported to show remarkable activity converting carbon dioxide into formate. Formate dehydrogenase 1 from M. extorquens AM1 (MeFDH1) was verified as the key responsible enzyme for the conversion of carbon dioxide to formate in this study. Using a 2% methanol concentration for induction, microbial harboring the recombinant MeFDH1 expressing plasmid produced the highest concentration of formate (26.6 mM within 21 hours) in electrochemical reactor. 60 μM of sodium tungstate in the culture medium was optimal for the expression of recombinant MeFDH1 and production of formate (25.7 mM within 21 hours). The recombinant MeFDH1 expressing cells showed maximum formate productivity of 2.53 mM/g-wet cell/hr, which was 2.5 times greater than that of wild type. Thus, M. extorquens AM1 was successfully engineered by expressing MeFDH1 as recombinant enzyme to elevate the production of formate from CO after elucidating key responsible enzyme for the conversion of CO to formate.
将二氧化碳转化为甲酸盐是构建 C1 化学平台的基本步骤。甲基杆菌 AM1 被报道具有将二氧化碳转化为甲酸盐的显著活性。在本研究中，甲基杆菌 AM1 的甲酸脱氢酶 1（MeFDH1）被证实是将二氧化碳转化为甲酸盐的关键负责酶。使用 2%甲醇浓度进行诱导，携带重组 MeFDH1 表达质粒的微生物在电化学反应器中产生了最高浓度的甲酸盐（21 小时内 26.6mM）。培养基中 60μM 的钨酸钠对重组 MeFDH1 的表达和甲酸盐的生产（21 小时内 25.7mM）最有利。表达重组 MeFDH1 的细胞的最大甲酸盐生产力为 2.53mM/g-湿细胞/小时，比野生型高 2.5 倍。因此，通过表达 MeFDH1 作为重组酶，成功地对甲基杆菌 AM1 进行了工程改造，提高了 CO 转化为甲酸盐的产量，阐明了 CO 转化为甲酸盐的关键负责酶。
Zotero 插件