National Key Laboratory of Wheat and Maize Crop Science/Collaborative Innovation Center of Henan Grain Crops/College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, China.
Sci Rep. 2018 May 8;8(1):7159. doi: 10.1038/s41598-018-23874-6.
Ear height is an important maize morphological trait that influences plant lodging resistance in the field, and is based on the number and length of internodes under the ear. To explore the effect of internodes on ear height, the internodes under the ear were analysed in four commercial hybrids (Jinsai6850, Zhengdan958, Xundan20, and Yuyu22) from different heterotic groups in China. The eighth internode, which is the third aboveground extended internode, exhibited high-parent or over high-parent heterosis and contributed considerably to ear height. Thus, the proteome of the eighth internode was examined. Sixty-six protein spots with >1.5-fold differences in accumulation (P < 0.05) among the four hybrids were identified by mass spectrometry and data analyses. Most of the differentially accumulated proteins exhibited additive accumulation patterns, but with epistatic effects on heterosis performance. Proteins involved in phenylpropanoid and benzoxazinoid metabolic pathways were observed to influence indole-3-acetic acid biosynthesis and polar auxin transport during internode development. Moreover, indole-3-acetic acid content was positively correlated with the eighth internode length, but negatively correlated with the extent of the heterosis of the eighth internode length.
穗位高是一个重要的玉米形态学性状，影响田间抗倒伏性，它基于穗下节间的数量和长度。为了探讨节间对穗位高的影响，对来自中国不同杂种优势群的四个商业杂交种（金赛 6850、郑单 958、旬单 20 和豫玉 22）的穗下节间进行了分析。第八节间，即第三地上伸长节间，表现出高亲或超高亲杂种优势，对穗位高有很大贡献。因此，对第八节间的蛋白质组进行了研究。通过质谱分析和数据分析，鉴定出四个杂交种之间积累差异大于 1.5 倍（P < 0.05）的 66 个蛋白点。大多数差异积累蛋白表现出累加模式，但对杂种优势表现有上位性效应。观察到参与苯丙烷和苯并恶嗪类代谢途径的蛋白质影响吲哚-3-乙酸生物合成和极性生长素运输在节间发育过程中。此外，吲哚-3-乙酸含量与第八节间长度呈正相关，与第八节间长度杂种优势的程度呈负相关。
