State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, People's Republic of China.
J Proteome Res. 2012 Apr 6;11(4):2492-507. doi: 10.1021/pr2011878. Epub 2012 Mar 21.
In natural conditions, culms of developing Moso bamboo, Phyllostachys heterocycla var. pubescens, reach their final height of more than ten meters within a short period of two to four months. To study this phenomenon, bamboo culm material collected from different developmental stages and internodes was analyzed. Histological observations indicated that the development of culm was dominated by cell division in the initial stages and by cell elongation in the middle and late stages. Development, maturation, and aging in different regions of the culm were studied systematically from the basal to the top internode. The four major endogenous hormones, indole acetic acid, gibberellic acid, zeatin riboside, and abscisic acid appeared to strongly influence the cell elongation phase. A total of 258 spots were differentially expressed in culm development. Of these, 213 spots were identified by MALDI-TOF/TOF MS and were involved in many physiological and metabolic processes including carbohydrate metabolism, cell division, cell expansion, protein synthesis, amino acid metabolism and redox homeostasis. These proteins with different expression patterns constructed an ingenious network to regulate the culm development. Developmental stage-specific and internode-specific protein expression patterns were identified. Protein abundance was regulated temporally and to some extent spatially, and the sequential development from base to apex of bamboo culm was implemented by temporal and spatial expression of enzymes. Results indicate that during development energy was mainly derived from sucrose degradation, as photosynthetic capacity was poor. The regulation of anaerobic and aerobic modes of respiration appeared to play an important role in energy generation. This is the first report on proteomic profiling in bamboo and helps in understanding the regulatory processes in developing culms.
在自然条件下，毛竹（Phyllostachys heterocycla var. pubescens）的茎干在两到四个月的短时间内就能长到十多米高。为了研究这一现象，我们对取自不同发育阶段和节间的竹秆材料进行了分析。组织学观察表明，茎干的发育最初阶段以细胞分裂为主，中期和后期以细胞伸长为主。我们系统地研究了竹秆不同区域从基部到顶部节间的发育、成熟和衰老过程。四种主要的内源性激素（吲哚乙酸、赤霉素、玉米素核苷和脱落酸）似乎强烈影响着细胞伸长阶段。在竹秆发育过程中，共检测到 258 个差异表达的斑点。其中，通过 MALDI-TOF/TOF MS 鉴定了 213 个斑点，这些斑点参与了许多生理和代谢过程，包括碳水化合物代谢、细胞分裂、细胞扩展、蛋白质合成、氨基酸代谢和氧化还原稳态。这些具有不同表达模式的蛋白质构建了一个巧妙的网络来调节茎干发育。鉴定到了发育阶段特异性和节间特异性的蛋白质表达模式。蛋白质丰度在时间和一定程度上的空间上受到调节，并且通过酶的时空表达实现了从竹秆基部到顶部的顺序发育。结果表明，在发育过程中，能量主要来自蔗糖的降解，因为光合能力较差。无氧和有氧呼吸模式的调节似乎在能量产生中起着重要作用。这是关于竹子蛋白质组学分析的首次报道，有助于理解发育中茎干的调控过程。
