Ye Fei, Gong Yi, Chen Xiangheng, Yu Meiying, Zuo Zhongkun, Pei Dongni, Liu Wei, Wang Qunwei, Zhou Jun, Duan Lunxi, Zhang Leiyi, Li Xiaojing, Tang Tenglong, Huang Jiangsheng
Department of Minimally Invasive Surgery, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China.
Oncol Lett. 2018 May;15(5):7523-7530. doi: 10.3892/ol.2018.8266. Epub 2018 Mar 15.
Long noncoding RNA serves important roles in gastric cancer (GC). However, the prognostic significance and tumorigenesis effect of AFAP1-antisense RNA 1 (AS1) in GC remain to be clarified. The present study was conducted in order to determine the expression level of AFAP1-AS1 by reverse transcription-quantitative polymerase chain reaction. It was demonstrated that AFAP1-AS1 expression level was higher in GC tissues in comparison with adjacent tissues. By analyzing 66 GC tissue specimens, AFAP1-AS1 expression level was found to be markedly associated with tumor size, clinical stage and differentiation. By performing multivariate Cox regression test, AFAP1-AS1 expression level was confirmed to be an independent factor for poor prognosis in patients with GC. Furthermore, SGC-7901 and BGC-823 cells were used for further investigation following transfection of an AFAP1-AS1 short hairpin RNA lentiviral vector. Knockdown of AFAP1-AS1 significantly inhibited GC cell proliferation, migration and invasion abilities . Finally, nude mice experiments confirmed that downregulation of AFAP1-AS1 in GC cells suppressed tumor growth . In conclusion, the results of the present study suggested that AFAP1-AS1 may serve as a valuable prognostic indicator and therapeutic target for GC.
长链非编码RNA在胃癌（GC）中发挥重要作用。然而，AFAP1反义RNA 1（AS1）在GC中的预后意义和肿瘤发生作用仍有待阐明。本研究旨在通过逆转录定量聚合酶链反应确定AFAP1-AS1的表达水平。结果表明，与相邻组织相比，GC组织中AFAP1-AS1的表达水平更高。通过分析66例GC组织标本，发现AFAP1-AS1表达水平与肿瘤大小、临床分期和分化程度显著相关。通过多因素Cox回归检验，证实AFAP1-AS1表达水平是GC患者预后不良的独立因素。此外，在转染AFAP1-AS1短发夹RNA慢病毒载体后，使用SGC-7901和BGC-823细胞进行进一步研究。敲低AFAP1-AS1可显著抑制GC细胞的增殖、迁移和侵袭能力。最后，裸鼠实验证实，GC细胞中AFAP1-AS1的下调可抑制肿瘤生长。总之，本研究结果表明，AFAP1-AS1可能是GC的一个有价值的预后指标和治疗靶点。
