Umesono K, Inokuchi H, Shiki Y, Takeuchi M, Chang Z, Fukuzawa H, Kohchi T, Shirai H, Ohyama K, Ozeki H
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
J Mol Biol. 1988 Sep 20;203(2):299-331. doi: 10.1016/0022-2836(88)90002-2.
The nucleotide sequence (56,410 base-pairs) of the large single-copy region of chloroplast DNA from the liverwort Marchantia polymorpha has been determined. The sequence starts from one end (JLA) of the large single-copy region and encompasses genes for 21 tRNAs, six ATPase subunits (atpA, atpB, atpE, atpF, atpH and atpI), two photosystem I polypeptides (psaA and psaB), four photosystem II polypeptides (psbA, psbC, psbD and psbG), five ribosomal proteins (rps2, rps4, rps7, rps'12 and rps14), and three RNA polymerase subunits (rpoB, rpoC1 and rpoC2). In addition, we detected 18 open reading frames ranging from 29 to 2136 amino acid residues long, four of which share significant amino acid sequence homology to those of an Escherichia coli malK protein (designated mbpX), human mitochondrial ND2 (ndh2) and ND3 (ndh3) of a respiratory chain NADH dehydrogenase, or a bacterial antenna protein of a light-harvesting complex (lhcA). Sequence analysis suggests that four tRNA genes and six protein genes might be split by introns; they are trnG(UCC), trnK(UUU), trnL(UAA), trnV(UAC), atpF, ndh2, rpoC1, rps'12, ORF135 and ORF167. In the large single-copy region described here, the gene organization deduced is highly conserved with respect to that of higher plants, but an inversion of some 30,000 base-pairs flanked by trnL(CAA) and trnD(GUC) was seen between the liverwort and tobacco chloroplast genomes.
已测定了地钱多歧苔叶绿体DNA大单拷贝区的核苷酸序列（56,410个碱基对）。该序列从大单拷贝区的一端（JLA）开始，包含21个tRNA、六个ATP酶亚基（atpA、atpB、atpE、atpF、atpH和atpI）、两个光系统I多肽（psaA和psaB）、四个光系统II多肽（psbA、psbC、psbD和psbG）、五个核糖体蛋白（rps2、rps4、rps7、rps12和rps14）以及三个RNA聚合酶亚基（rpoB、rpoC1和rpoC2）的基因。此外，我们检测到18个开放阅读框，长度从29到2136个氨基酸残基不等，其中四个与大肠杆菌malK蛋白（命名为mbpX）、人类线粒体呼吸链NADH脱氢酶的ND2（ndh2）和ND3（ndh3）或光捕获复合体的细菌天线蛋白（lhcA）具有显著的氨基酸序列同源性。序列分析表明，四个tRNA基因和六个蛋白质基因可能被内含子分割；它们是trnG(UCC)、trnK(UUU)、trnL(UAA)、trnV(UAC)、atpF、ndh2、rpoC1、rps12、ORF135和ORF167。在此描述的大单拷贝区中，推导的基因组织与高等植物的基因组织高度保守，但在地钱和烟草叶绿体基因组之间，发现了一段约30,000个碱基对的倒位，其两侧为trnL(CAA)和trnD(GUC)。
