Kohchi T, Shirai H, Fukuzawa H, Sano T, Komano T, Umesono K, Inokuchi H, Ozeki H, Ohyama K
Research Centre for Cell and Tissue Culture, Kyoto University, Japan.
J Mol Biol. 1988 Sep 20;203(2):353-72. doi: 10.1016/0022-2836(88)90004-6.
We characterized the genes in the regions of large inverted repeats (IRA and IRB, 10,058 base-pairs each) and a small single copy (SSC 19,813 bp) of chloroplast DNA from Marchantia polymorpha. The inverted repeat (IR) regions contain genes for four ribosomal RNAs (16 S, 23 S, 4.5 S and 5 S rRNAs) and five transfer RNAs (valine tRNA(GAC), isoleucine tRNA(GAU), alanine tRNA(UGC), arginine tRNA(ACG) and asparagine tRNA(GUU)). The gene organization of the IR regions in the liverwort chloroplast genome is conserved, although the IR regions are smaller (10,058 base-pairs) than any reported in higher plant chloroplasts. The small single-copy region (19,813 base-pairs) encoded genes for 17 open reading frames, a leucine tRNA(UAG) and a proline tRNA(GGG)-like sequence. We identified 12 open reading frames by homology of their coding sequences to a 4Fe-4S-type ferredoxin protein, a bacterial nitrogenase reductase component (Fe-protein), five human mitochondrial components of NADH dehydrogenase (ND1, ND4, ND4L, ND5 and ND6), two Escherichia coli ribosomal proteins (S15 and L21), two putative proteins encoded in the kinetoplast maxicircle DNA of Leishmania tarentolae (LtORF 3 and LtORF 4), and a bacterial permease inner membrane component (encoded by malF in E. coli or hisQ in Salmonella typhimurium).
我们对来自多歧苏铁叶绿体DNA的大反向重复序列（IRA和IRB，各10,058个碱基对）和小单拷贝序列（SSC，19,813 bp）区域中的基因进行了表征。反向重复（IR）区域包含四种核糖体RNA（16 S、23 S、4.5 S和5 S rRNA）和五种转移RNA（缬氨酸tRNA(GAC)、异亮氨酸tRNA(GAU)、丙氨酸tRNA(UGC)、精氨酸tRNA(ACG)和天冬酰胺tRNA(GUU)）的基因。尽管地钱叶绿体基因组中的IR区域（10,058个碱基对）比高等植物叶绿体中报道的任何区域都小，但其基因组织是保守的。小单拷贝区域（19,813个碱基对）编码了17个开放阅读框、一个亮氨酸tRNA(UAG)和一个类似脯氨酸tRNA(GGG)的序列。我们通过将其编码序列与4Fe-4S型铁氧化还原蛋白、细菌固氮酶还原酶组分（Fe蛋白）、人类线粒体NADH脱氢酶的五个组分（ND1、ND4、ND4L、ND5和ND6）、两种大肠杆菌核糖体蛋白（S15和L21）、利什曼原虫动质体大环DNA中编码的两种假定蛋白（LtORF 3和LtORF 4）以及细菌通透酶内膜组分（在大肠杆菌中由malF编码，在鼠伤寒沙门氏菌中由hisQ编码）进行同源性比较，鉴定出了12个开放阅读框。
