Fukuzawa H, Kohchi T, Sano T, Shirai H, Umesono K, Inokuchi H, Ozeki H, Ohyama K
Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, Japan.
J Mol Biol. 1988 Sep 20;203(2):333-51. doi: 10.1016/0022-2836(88)90003-4.
The nucleotide sequence (25,320 base-pairs) of a part of the large single-copy region of chloroplast DNA from the liverwort Marchantia polymorpha was determined. This region encodes putative genes for four tRNAs, isoleucine tRNA(CAU), arginine tRNA(CCG), proline tRNA(UGG) and tryptophan tRNA(CCA); eight photosynthetic polypeptides, the large subunit of ribulose bisphosphate carboxylase/oxygenase (rbcL), 51,000 Mr photosystem II chlorophyll alpha apoprotein (psbB), apocytochrome b-559 polypeptides (psbE and psbF), 10,000 Mr phosphoprotein (psbH), cytochrome f preprotein (petA), cytochrome b6 polypeptide (petB), and cytochrome b6/f complex subunit 4 polypeptide (petD); 13 ribosomal proteins (L2, L14, L16, L20, L22, L23, L33, S3, S8, S11, S12, S18 and S19); initiation factor 1 (infA); ribosome-associating polypeptide (secX); and alpha subunit of RNA polymerase (rpoA). Functionally related genes were located in several clusters in this region of the genome. There were two ribosomal protein gene clusters: rpl23-rpl2-rps19-rpl22-rps3-rpl16-+ ++rpl14-rps8-infA-secX-rps11-rpoA, with a gene arrangement similar to that of the Escherichia coli S10-spc-alpha operons, and the rps12'-rpl20-rps18-rpl33 cluster. There were gene clusters encoding photosynthesis components such as the psbB-psbH-petB-petD and the psbE-psbF clusters. Thirteen open reading frames, ranging in length from 31 to 434 amino acid residues, remain to be identified.
测定了苔类植物多歧银叶苔叶绿体DNA大单拷贝区一部分的核苷酸序列（25,320个碱基对）。该区域编码四个tRNA的推定基因，即异亮氨酸tRNA（CAU）、精氨酸tRNA（CCG）、脯氨酸tRNA（UGG）和色氨酸tRNA（CCA）；八个光合多肽，即核酮糖二磷酸羧化酶/加氧酶的大亚基（rbcL）、51,000道尔顿的光系统II叶绿素α脱辅基蛋白（psbB）、脱辅基细胞色素b - 559多肽（psbE和psbF）、10,000道尔顿的磷蛋白（psbH）、细胞色素f前体蛋白（petA）、细胞色素b6多肽（petB）以及细胞色素b6/f复合体亚基4多肽（petD）；13个核糖体蛋白（L2、L14、L16、L20、L22、L23、L33、S3、S8、S11、S12、S18和S19）；起始因子1（infA）；核糖体相关多肽（secX）；以及RNA聚合酶的α亚基（rpoA）。功能相关的基因位于基因组的这一区域的几个簇中。有两个核糖体蛋白基因簇：rpl23 - rpl2 - rps19 - rpl22 - rps3 - rpl16 - +++rpl14 - rps8 - infA - secX - rps11 - rpoA，其基因排列与大肠杆菌S10 - spc - α操纵子相似，以及rps12' - rpl20 - rps18 - rpl33簇。有编码光合作用组件的基因簇，如psbB - psbH - petB - petD和psbE - psbF簇。13个开放阅读框，长度从31到434个氨基酸残基不等，有待鉴定。
